Li Y, G Zhang C, Wang X-H, Liu D-H
Department of Cardiology, Tangshan Worker Hospital, Tangshan, Hebei, China.
Eur Rev Med Pharmacol Sci. 2016 Sep;20(18):3863-3867.
To construct the atherosclerosis model of ApoE-knockout (ApoE-/-) mouse after deposition of high-fat diet at brachiocephalic artery and coronary artery.
40 healthy 8-week SPF-level male ApoE-/- mice of body weigh 16 to 23 g were averagely divided into a high-fat diet group and an ordinary diet group. Mice were sacrificed in a time course study by 3 months, 6 months, 9 months and 12 months. The brachiocephalic artery and coronary artery were extracted for pathological examination, and enzyme-linked immunosorbent assay (ELISA) was used for the examination of serum high-sensitivity C-reactive protein (hs-CRP) content and the relative expression level of liver peroxisome proliferator activated receptor (PPAR).
Plaque ratio in lumen area, lipid ratio in plaque area and plaque spontaneous rupture rate of brachiocephalic artery and coronary artery in high-fat diet group are all significantly higher than those in ordinary diet group during the same period (p<0.05). In the high-fat diet group, those indexes mentioned above in brachiocephalic artery are all significantly higher than those in coronary artery during the same period (p<0.05). Hs-CRP content in high-fat diet group is significantly higher than that in ordinary diet group during the same period, while PPAR relative expression level is significantly lower than that in ordinary diet group during the same period (p<0.05).
It was found that ApoE-/- mice after high-fat diet depict the phenotype of the atherosclerotic mouse model, and the brachiocephalic artery is better than coronary artery in terms of material selection, with inflammatory responses also playing an important role.
通过高脂饮食在头臂动脉和冠状动脉处沉积构建载脂蛋白E基因敲除（ApoE-/-）小鼠动脉粥样硬化模型。
选取40只体重16至23克、8周龄的健康SPF级雄性ApoE-/-小鼠，平均分为高脂饮食组和普通饮食组。在3个月、6个月、9个月和12个月的时间进程研究中处死小鼠。提取头臂动脉和冠状动脉进行病理检查，并采用酶联免疫吸附测定（ELISA）法检测血清高敏C反应蛋白（hs-CRP）含量及肝脏过氧化物酶体增殖物激活受体（PPAR）的相对表达水平。
高脂饮食组在同一时期头臂动脉和冠状动脉的管腔面积斑块率、斑块面积脂质率及斑块自发破裂率均显著高于普通饮食组（p<0.05）。在高脂饮食组中，同一时期头臂动脉上述指标均显著高于冠状动脉（p<0.05）。高脂饮食组同一时期hs-CRP含量显著高于普通饮食组，而PPAR相对表达水平显著低于普通饮食组（p<0.05）。
发现高脂饮食后的ApoE-/-小鼠呈现动脉粥样硬化小鼠模型的表型，在取材方面头臂动脉优于冠状动脉，且炎症反应也起重要作用。
