Fagerholm P, Chylack L T
Howe Laboratory, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston.
Acta Ophthalmol (Copenh). 1989 Feb;67(1):13-20. doi: 10.1111/j.1755-3768.1989.tb00717.x.
Paired rabbit lenses were kept in organ culture; one of the lenses was subjected to hypoglycemia, and the other served as control. The lens changes were followed and examined by photomacrography, light microscopy of the intact lens, and light and electron microscopy at different time intervals. The possibility of reversing the morphological changes was evaluated at different times by replacing the hypoglycemic medium with a normal glycemic one. The central zone of the lens epithelium was affected first through the formation of large extracellular vacuoles. These changes were reversible. Subsequently, the central lens epithelial cells showed severe swelling and breakdown of the lens membranes. These changes were not reversible. With time the epithelial changes spread from the central zone towards the equator. Lens fiber swelling followed the severe epithelial damage. These findings show that there is a regional sensitivity to hypoglycemia within the lens epithelium. The results are discussed in relation to hexokinase activity.
将配对的兔晶状体置于器官培养中；其中一个晶状体经历低血糖处理，另一个作为对照。通过宏观摄影、完整晶状体的光学显微镜检查以及在不同时间间隔进行光学显微镜和电子显微镜检查来跟踪和观察晶状体的变化。通过在不同时间用正常血糖培养基替换低血糖培养基来评估逆转形态变化的可能性。晶状体上皮的中央区首先受到影响，通过形成大的细胞外液泡。这些变化是可逆的。随后，晶状体中央上皮细胞出现严重肿胀和晶状体膜破裂。这些变化是不可逆的。随着时间的推移，上皮变化从中央区向赤道扩散。晶状体纤维肿胀发生在严重的上皮损伤之后。这些发现表明晶状体上皮内对低血糖存在区域敏感性。结合己糖激酶活性对结果进行了讨论。
