De Leon-Rodriguez Luis M, Hemar Yacine, Mo Guang, Mitra Alok K, Cornish Jillian, Brimble Margaret A
School of Biological Sciences, The University of Auckland, 3A Symonds St, Thomas Building, Auckland 1010, New Zealand.
School of Chemical Sciences, The University of Auckland, 23 Symonds St, Auckland 1010, New Zealand.
Acta Biomater. 2017 Jan 1;47:40-49. doi: 10.1016/j.actbio.2016.10.014. Epub 2016 Oct 12.
We report the synthesis and characterization of multifunctional peptides comprised of a hydrogel forming β-sheet peptide segment and a matrix metalloproteinase 2 substrate containing a propargylglycinyl linker that is further derivatized with an RGD peptide sequence via "click" chemistry. In contrast to currently known systems, these multifunctional peptides formed gels that are stiffer than those formed by their respective precursors. All the peptides showed reversible thermoresponsive properties, which render them as suitable lead systems for a variety of possible biomedical applications.
In general, it has been frequently observed that chemical biofunctionalization of peptide hydrogels adversely affects peptide assembly, hydrogel formation or mechanical properties, which severely compromises their application. A functionalization protocol that allows to generate peptide hydrogels that display significantly improved mechanical properties over their unfunctionalized counterparts is reported in this work. These peptides also showed thermoresponsive viscoelastic characteristics, including an example of a peptide hydrogel that displays lower critical solution temperature behaviour.
我们报道了多功能肽的合成与表征，该多功能肽由形成水凝胶的β-折叠肽段和含炔丙基甘氨酰接头的基质金属蛋白酶2底物组成，该接头经“点击”化学法进一步用RGD肽序列衍生化。与目前已知的体系相比，这些多功能肽形成的凝胶比其各自前体形成的凝胶更硬。所有肽均表现出可逆的热响应特性，使其成为各种可能生物医学应用的合适先导体系。
一般而言，经常观察到肽水凝胶的化学生物功能化会对肽组装、水凝胶形成或机械性能产生不利影响，这严重损害了它们的应用。本工作报道了一种功能化方案，该方案能够生成与未功能化对应物相比具有显著改善机械性能的肽水凝胶。这些肽还表现出热响应粘弹性特征，包括一个表现出较低临界溶液温度行为的肽水凝胶实例。
