小鼠鱼精蛋白1基因在转基因小鼠中的表达。

Expression of mouse protamine 1 genes in transgenic mice.

作者信息

Peschon J J, Behringer R R, Palmiter R D, Brinster R L

机构信息

Department of Biochemistry, University of Washington, Seattle 98195.

出版信息

Ann N Y Acad Sci. 1989;564:186-97. doi: 10.1111/j.1749-6632.1989.tb25897.x.

DOI:10.1111/j.1749-6632.1989.tb25897.x

PMID:2774416

Abstract

Mouse protamine genes are expressed exclusively in spermatids. Mouse protamine 1 (mP1) transcriptional regulatory elements can target the expression of either marked mP1 transgenes or mP1 chimeric genes to spermatids in transgenic mice. Sequences between -40 and -465 bp relative to the transcription start site are required for expression in spermatids, whereas sequences 3' of the point of translation initiation are dispensable. mP1 transcriptional regulatory sequences were used to direct the expression of a toxic gene product to spermatids. The phenotypic consequences of toxin expression in spermatids are described.

摘要

小鼠鱼精蛋白基因仅在精子细胞中表达。小鼠鱼精蛋白1（mP1）转录调控元件可将标记的mP1转基因或mP1嵌合基因的表达靶向转基因小鼠的精子细胞。相对于转录起始位点，-40至-465 bp之间的序列是在精子细胞中表达所必需的，而翻译起始点3'端的序列则是可有可无的。mP1转录调控序列被用于将一种毒性基因产物的表达导向精子细胞。本文描述了精子细胞中毒素表达的表型后果。