Macdonald Rachel, Wilson S W
Developmental Biology Research Centre, Randall Institute, Kings College London, 26-29 Drury Lane, London WC2B 5RL, UK, , , , , , GB.
Dev Genes Evol. 1997 Jan;206(6):363-369. doi: 10.1007/s004270050065.
Although Pax6 is required during eye development in rodents and humans, little is known about the precise role of the protein in this process. To aid in the interpretation of functional studies, we have determined the precise spatial and temporal distributions of the Pax6 protein in the eye. We find that Pax6 is initially distributed contiguously throughout a large domain of the anterior neural plate of zebrafish, including the presumptive eye fields and the dorsal diencephalon. After evagination of the optic vesicle, Pax6 becomes restricted to all proliferating cells of the pigment epithelial and neural layers of the retina. Pax6 is downregulated in most cells concomitant with differentiation. However, it remains present in several mature cell types of the eye including amacrine cells and the lens and corneal epithelia. This expression is conserved across diverse vertebrate species and suggests that Pax6 has additional conserved functions in the mature eye.
尽管在啮齿动物和人类的眼睛发育过程中需要Pax6，但对于该蛋白质在此过程中的精确作用知之甚少。为了有助于对功能研究的解释，我们已经确定了Pax6蛋白在眼睛中的精确空间和时间分布。我们发现，Pax6最初连续分布在斑马鱼前神经板的一个大区域，包括假定的眼区和背侧间脑。视泡外翻后，Pax6局限于视网膜色素上皮和神经层的所有增殖细胞。Pax6在大多数伴随分化的细胞中下调。然而，它仍存在于眼睛的几种成熟细胞类型中，包括无长突细胞以及晶状体和角膜上皮。这种表达在不同的脊椎动物物种中是保守的，表明Pax6在成熟眼睛中具有额外的保守功能。
