Amano H, Koizumi Osamu, Kobayakawa Y
Faculty of Human Environmental Science, Fukuoka Women's University, Higashi-ku, Fukuoka 813, Japan, , , , , , JP.
Department of Biology, Faculty of Science, Kyushu University, Chuo-ku, Fukuoka 810, Japan, , , , , , JP.
Dev Genes Evol. 1997 Dec;207(6):413-416. doi: 10.1007/s004270050131.
We produced a monoclonal antibody, AE03, which recognized mucous granules in the basal disk gland cells in Hydra and the secreted mucus with which they stick onto substrate. AE03 also recognized atrichous isorhizas, one of the four types of nematocyst present in tentacles, and their nematoblasts present in the body column. With this monoclonal antibody, we could observe the detailed morphogenesis of the atrichous isorhiza from the beginning of its formation. The elongation and invagination processes of external tubes and correspondence between the external tubes and the thread of discharged nematocysts were confirmed.
我们制备了一种单克隆抗体AE03，它能识别水螅基盘腺细胞中的黏液颗粒以及它们分泌的用于附着在基质上的黏液。AE03还能识别触手处四种刺丝囊类型之一的无刺等丝囊及其体柱中的刺细胞母细胞。利用这种单克隆抗体，我们可以从无刺等丝囊形成之初观察到其详细的形态发生过程。证实了外管的伸长和内陷过程以及外管与排出刺丝囊的丝之间的对应关系。
