Gong Chengcheng, Chen Tao, Chen Huifeng, Zhang Shanshan, Wang Xinzhu, Wang Weiqing, Sun Jing, Li Yulin, Liao Zhixin
School of Chemistry and Chemical Engineering, Southeast University, Nanjing, P.R. China.
Key Laboratory of Tibetan medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, P.R. China.
J Sep Sci. 2016 Dec;39(23):4660-4666. doi: 10.1002/jssc.201600805. Epub 2016 Nov 9.
In this paper, we report an efficient method by high-speed counter-current chromatography for the first separation of four aromatic acids and two analogs with similar structures and polarities from Clematis akebioides. First, the ethyl acetate extract was treated by silica gel column chromatography to enrich the target compounds. And then the fraction with target compounds were purified by high-speed counter-counter chromatography using a two-phase solvent system consisting of chloroform/acetonitrile/water (10:6:4, v/v). The results showed high-speed counter-current chromatography could be a powerful technology for the separation of compounds with similar structures and polarities. Besides, it was found acetonitrile could be a good methanol substitute when a chloroform/methanol/water system could not provide a good separation factor. This study provides a reference for the separation of compounds from Clematis akebioides.
在本文中，我们报道了一种高效的方法，即通过高速逆流色谱法首次从毛茛铁线莲中分离出四种芳香酸和两种结构及极性相似的类似物。首先，将乙酸乙酯提取物用硅胶柱色谱法处理以富集目标化合物。然后，使用由氯仿/乙腈/水（10:6:4，v/v）组成的两相溶剂系统，通过高速逆流色谱法对含有目标化合物的馏分进行纯化。结果表明，高速逆流色谱法可能是分离结构和极性相似化合物的有力技术。此外，还发现当氯仿/甲醇/水系统不能提供良好的分离因子时，乙腈可以作为甲醇的良好替代品。本研究为从毛茛铁线莲中分离化合物提供了参考。
