Arfuso F, Guerrera M C, Fortino G, Fazio F, Santulli A, Piccione G
Department of Veterinary Sciences, University of Messina, Polo Universitario dell'Annunziata, Messina, Italy.
Acqua Azzurra S.p.A., Contrada Morghella, Pachino, Italy.
Theriogenology. 2017 Jan 15;88:145-151. doi: 10.1016/j.theriogenology.2016.09.028. Epub 2016 Sep 21.
The effect of rearing temperature on gonad differentiation and growth was evaluated in sea bass (Dicentrarchus labrax). One control group (CG, n = 60) and two experimental groups (EG1, n = 30; EG2, n = 30) were selected. CG was reared at 15.0 °C during 10 days post-hatch (dph) and at 19.0 °C throughout the remaining larval and post-larval development. EG1 was exposed to 14.5 °C from 1 to 50 dph, followed by an increase to 20 °C until sampling (176 dph). EG2 was exposed to 14.5 °C from 1 to 37 dph, followed by an increase to 20 °C until sampling (226 dph); 30 fish from CG were randomly sampled at 176 dph (CG1, control for EG1) and 30 fish were sampled at 226 dph (CG2, control for EG2). Weight, total and fork length measurement, and gonad collection were performed. All biometric indices were higher in EG1 and EG2 than in CG1 and CG2, respectively. Histologic analysis reported 100% of undifferentiated gonads in CG1 and; EG1 reported 80% of undifferentiated gonads and 20% of gonads at early differentiation toward the male line. CG2 reported 34% of undifferentiated gonads, 10% of gonads at early differentiation toward the female line, 23% of gonads at early differentiation toward the male line, and 33% of gonads with intratesticular oocytes. EG2 reported 47% of gonads differentiated in testis, 33% gonads differentiated in ovary, and 20% of gonads with intratesticular oocytes. These results suggest that water temperature plays an important role in the process of gonad differentiation and growth of sea bass.
在海鲈（欧洲鲈）中评估了养殖温度对性腺分化和生长的影响。选取了一个对照组（CG，n = 60）和两个实验组（EG1，n = 30；EG2，n = 30）。对照组在孵化后10天（dph）期间饲养在15.0°C，在整个剩余的幼体和幼体后发育阶段饲养在19.0°C。EG1在1至50 dph期间暴露于14.5°C，随后温度升高至20°C直至取样（176 dph）。EG2在1至37 dph期间暴露于14.5°C，随后温度升高至20°C直至取样（226 dph）；在176 dph时从对照组随机抽取30条鱼（CG1，作为EG1的对照），在226 dph时抽取30条鱼（CG2，作为EG2的对照）。进行了体重、全长和叉长测量以及性腺采集。所有生物测量指标在EG1和EG2中分别高于CG1和CG2。组织学分析报告CG1中100%为未分化性腺；EG1报告80%为未分化性腺，20%为早期向雄性系分化的性腺。CG2报告34%为未分化性腺，10%为早期向雌性系分化的性腺，23%为早期向雄性系分化的性腺，33%为具有睾丸内卵母细胞的性腺。EG2报告47%的性腺分化为睾丸，33%的性腺分化为卵巢，20%的性腺具有睾丸内卵母细胞。这些结果表明水温在海鲈性腺分化和生长过程中起重要作用。
