Mhalhel Kamel, Arena Rosaria, Rizzo Maria, Piccione Giuseppe, Aragona Marialuisa, Levanti Maria, Aragona Francesca, Arfuso Francesca
Department of Veterinary Sciences, University of Messina, Viale Giovanni Palatucci SNC, 98168 Messina, Italy.
Marine Biochemistry and Ecotoxicology Laboratory, Department of Earth and Sea Science, University of Palermo, Via Barlotta 4, 91100 Trapani, Italy.
Animals (Basel). 2024 Mar 27;14(7):1024. doi: 10.3390/ani14071024.
In this study, the expression and implication of acid-sensing ion channels 2 and 4 (ASIC2 and ASIC4) in the gonadal sex differentiation of (), subjected to increasing water temperatures during gonadal development, were evaluated. Two groups were selected: a control group (CG), in which the average water temperature was maintained at 15 °C and increased to 20 °C in 20 days until weaning; and an experimental group (EG), in which the water temperature was retained at 15 °C for 60 days; thereafter, the temperature was increased daily by 0.5 °C until it reached 20 °C up to the weaning time. Ten fish from the CG and 13 fish from the EG were sampled randomly on the 335th day after hatching (dph). A higher percentage of gonad differentiation in ovaries rather than in testes was observed in the EG compared to the CG ( = 0.01). ASIC2 and ASIC4 were detected for the first time in ovaries by indirect immunofluorescence. Both ASIC2 and ASIC4 were expressed in previtellogenic oocytes of ovaries and in scattered cells within some testes, and were most likely intratesticular previtellogenic oocytes in both the CG and EG groups. The CG group showed a higher expression of ASIC4 than the EG cohort ( < 0.05). The results gathered in this study revealed the capacity of water temperature to influence both gonadal differentiation and growth in this gonochoristic fish species, and suggests the possible role of ASIC2 and ASIC4 in gonad differentiation and gamete development in .
在本研究中，对性腺发育过程中水温升高的（某物种）性腺性别分化过程中酸敏感离子通道2和4（ASIC2和ASIC4）的表达及意义进行了评估。选取了两组：对照组（CG），平均水温维持在15℃，并在20天内升至20℃直至断奶；实验组（EG），水温在60天内保持在15℃；此后，每天升温0.5℃，直至断奶时达到20℃。在孵化后第335天（dph），从CG组随机抽取10条鱼，从EG组随机抽取13条鱼。与CG组相比，EG组中卵巢性腺分化的比例高于睾丸（P = 0.01）。通过间接免疫荧光首次在（某物种）卵巢中检测到ASIC2和ASIC4。ASIC2和ASIC4均在卵巢的卵黄生成前卵母细胞以及部分睾丸中的散在细胞中表达，在CG组和EG组中很可能均为睾丸内卵黄生成前卵母细胞。CG组中ASIC4的表达高于EG组（P < 0.05）。本研究结果揭示了水温对这种雌雄异体鱼类性腺分化和生长的影响能力，并表明ASIC2和ASIC4在（某物种）性腺分化和配子发育中可能发挥的作用。
