Tominaga S, Tominaga K
Okinaka Memorial Institute for Medical Research, Tokyo, Japan.
Biochem J. 1989 Jul 1;261(1):57-61. doi: 10.1042/bj2610057.
Treatment of Ehrlich ascites-tumour (EAT) cells with interferon (IFN) abolished their ability to secrete a 32 kDa protein that was secreted by growing EAT cells. These IFN-treated cells secreted two proteins (molecular masses 100 and 89 kDa as estimated by SDS/polyacrylamide-gel electrophoresis) that were not detected in two-dimensional gel electrophoresis of the culture fluid of untreated EAT cells. The sequence of 20 amino acids from the N-terminal end of the 32 kDa protein was very similar to portions of sequences of mouse proviral gag proteins.
用干扰素（IFN）处理艾氏腹水瘤（EAT）细胞后，这些细胞分泌一种32 kDa蛋白质的能力丧失，而处于生长状态的EAT细胞能够分泌这种蛋白质。这些经干扰素处理的细胞分泌了两种蛋白质（通过SDS/聚丙烯酰胺凝胶电泳估计分子量分别为100 kDa和89 kDa），在未处理的EAT细胞培养液的二维凝胶电泳中未检测到这两种蛋白质。32 kDa蛋白质N端的20个氨基酸序列与小鼠前病毒gag蛋白的部分序列非常相似。
