编码高度保守的核仁磷蛋白B23的人类cDNA的核苷酸序列。

The nucleotide sequence of a human cDNA encoding the highly conserved nucleolar phosphoprotein B23.

作者信息

Li X Z, McNeilage L J, Whittingham S

机构信息

Burnet Clinical Research Unit, Walter and Eliza Hall Institute of Medical Research, Victoria, Australia.

出版信息

Biochem Biophys Res Commun. 1989 Aug 30;163(1):72-8. doi: 10.1016/0006-291x(89)92100-1.

DOI:10.1016/0006-291x(89)92100-1

PMID:2775293

Abstract

A cDNA clone containing the complete coding sequence for the human nucleolar phosphoprotein B23 was isolated from a Burkitt's lymphoma cDNA library by immunoscreening with human autoantibodies. The B23 clone contained a 1.3 kb cDNA insert encoding a polypeptide of 294 amino acids with a predicted molecular mass of 32,539 daltons. The deduced B23 amino acid sequence contained 2 acidic domains rich in aspartic and glutamic acid, a feature shared by a number of nuclear and nucleolar proteins. The human B23 amino acid sequence showed 98% homology with rat B23 and 68% homology with the Xenopus laevis nucleolar phosphoprotein, NO38 showing that the primary structure of B23 is highly conserved among these species.

摘要

通过用人自身抗体进行免疫筛选，从伯基特淋巴瘤cDNA文库中分离出一个包含人类核仁磷蛋白B23完整编码序列的cDNA克隆。B23克隆含有一个1.3kb的cDNA插入片段，编码一个由294个氨基酸组成的多肽，预测分子量为32539道尔顿。推导的B23氨基酸序列包含2个富含天冬氨酸和谷氨酸的酸性结构域，这是许多核蛋白和核仁蛋白共有的特征。人类B23氨基酸序列与大鼠B23的同源性为98%，与非洲爪蟾核仁磷蛋白NO38的同源性为68%，表明B23的一级结构在这些物种中高度保守。