Di Giulio A, Oratore A, Saletti M A, Tozzi-Ciancarelli M G, Crifò C
Dept. of Biomedical Sciences, University of L'Aquila, Italy.
Biochem Int. 1989 Jul;19(1):19-25.
The protoporphyrin IX (PPIX)-sensitized hemolysis of erythrocytes depleted of cholesterol was investigated. From 20% to 30% of the total membrane cholesterol was removed from cells by incubation with old autologous plasma or by means of interaction with L-alpha-phosphatidylcholine dipalmitoyl (DPPC) liposomes. As expected, after this treatment, the cells show an overall increase in membrane fluidity revealed by means of specific fluorescent probes. The same cells are more susceptible to the photohemolysis induced by PPIX excited by visible light, but gave no lysis in the absence of the sensitizer. As a consequence, the primary oxidative damage which is produced during irradiation can be possibly assigned to the phospholipidic and/or proteic moiety instead of the steroidal moiety.
研究了原卟啉IX（PPIX）致敏的胆固醇耗尽红细胞的溶血情况。通过与陈旧自体血浆孵育或与L-α-二棕榈酰磷脂酰胆碱（DPPC）脂质体相互作用，从细胞中去除了20%至30%的总膜胆固醇。正如预期的那样，经过这种处理后，通过特定荧光探针显示细胞的膜流动性总体增加。相同的细胞对可见光激发的PPIX诱导的光溶血更敏感，但在没有敏化剂的情况下不会发生裂解。因此，辐照过程中产生的主要氧化损伤可能归因于磷脂和/或蛋白质部分，而不是甾体部分。
