Lofton S A, Gown A M, Vogel A M, Krieger J N
Department of Pathology, University of Washington School of Medicine, Seattle.
Urology. 1989 May;33(5):433-9. doi: 10.1016/0090-4295(89)90045-9.
Definitive diagnosis of poorly differentiated and metastatic neoplasms may be impossible using conventional histologic criteria. Recent developments in cell biology and immunology now enable us to answer such difficult diagnostic problems. Several varieties of structural proteins can be identified in malignant cells using monoclonal antibodies. The composition of these proteins can yield information regarding the origin of a neoplasm. Intermediate filaments are one such family of structural proteins. By characterization of these proteins, using a panel of monoclonal antibodies, poorly differentiated tumors may be definitively classified as carcinomas, sarcomas, lymphomas, or neural tumors. This approach to tumor diagnosis is now applicable to difficult problems in clinical urology.
使用传统组织学标准可能无法对低分化和转移性肿瘤进行明确诊断。细胞生物学和免疫学的最新进展使我们现在能够回答此类棘手的诊断问题。使用单克隆抗体可以在恶性细胞中识别出几种结构蛋白。这些蛋白质的组成可以提供有关肿瘤起源的信息。中间丝就是这样一类结构蛋白。通过使用一组单克隆抗体对这些蛋白质进行表征，低分化肿瘤可以明确分类为癌、肉瘤、淋巴瘤或神经肿瘤。这种肿瘤诊断方法现在适用于临床泌尿外科的难题。
