Orraca Z M, Ríos-Olivares E, Carrasco-Canales J A, Aquino E, Marchand A M, Colón J I
Bol Asoc Med P R. 1989 Jul;81(7):254-8.
Natural killer (NK) cell activity and adoptive immunotherapy were used to characterize the anticancerous effect of a polyantigenic immunomodulator (PAI). PAI consists of a mixture of inactivated bacteria and influenza virus in a peanut oil-arlacel A-aluminum monoesterate emulsion, shown previously to have antitumoral activity in mice implanted with Ehrlich's ascites tumor. The administration of PAI, its bacterial or viral component strongly increased the in vitro activity of NK cells of splenocyte populations obtained from Swiss-Webster (allogeneic) and C57BL/6J (syngeneic) mice, specially during the early post-induction period. On the other hand, PAI-sensitized, allogeneic or syngeneic lymphocytes were transferred successfully to tumor-bearing mice implanted with Ehrlich's ascites tumor, reducing tumor growth and increasing survival. The results confirm our previous suggestions that PAI acts probably at the level of cellular immunity. Therefore complex polyantigenic substances such as PAI could be used directly alone, in combination with other immunoadjuvants or to sensitize in a global manner immunocompetent cells to be employed in adoptive immunotherapy.
自然杀伤（NK）细胞活性和过继性免疫疗法被用于表征一种多抗原免疫调节剂（PAI）的抗癌作用。PAI由灭活细菌和流感病毒在花生油-阿拉塞A-单硬脂酸铝乳液中的混合物组成，先前已证明其对接种艾氏腹水瘤的小鼠具有抗肿瘤活性。PAI、其细菌或病毒成分的给药显著增强了从瑞士-韦伯斯特（同种异体）和C57BL/6J（同基因）小鼠获得的脾细胞群体中NK细胞的体外活性，特别是在诱导后的早期阶段。另一方面，PAI致敏的同种异体或同基因淋巴细胞被成功转移到接种艾氏腹水瘤的荷瘤小鼠体内，减少了肿瘤生长并延长了生存期。结果证实了我们之前的推测，即PAI可能在细胞免疫水平起作用。因此，像PAI这样的复合多抗原物质可以直接单独使用，与其他免疫佐剂联合使用，或以整体方式使免疫活性细胞致敏，用于过继性免疫疗法。
