Varga Viktória E, Lőrincz Hajnalka, Zsíros Noémi, Fülöp Péter, Seres Ildikó, Paragh György, Balla József, Harangi Mariann
Department of Internal Medicine, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, H-4032, Debrecen, Hungary.
Lipids Health Dis. 2016 Oct 18;15(1):182. doi: 10.1186/s12944-016-0353-x.
Selective low-density lipoprotein (LDL) apheresis is commonly used to treat patients with familial hypercholesterolemia (FH). Chemerin is an adipokine with putative roles in the regulation of lipid metabolism.
In our pilot study, we measured serum chemerin levels by enzyme-linked immunosorbent assay in six severe heterozygous FH patients before and after their first LDL apheresis treatments using the technique of direct adsorption of lipoproteins (DALI).
The first treatment sessions decreased serum chemerin levels by an average of 27.26 %. While following one patient, 12 months of regular LDL apheresis resulted in a permanent reduction in his serum chemerin level. Changes in the lipoprotein subfractions measured by gel electrophoresis (Lipoprint) correlated with the reduction of chemerin levels. Furthermore, we eluted and then measured chemerin bound to the DALI column.
We conclude that LDL apheresis decreases the circulating level of chemerin by binding the protein to the column and thus improves lipoprotein subfraction pattern.
选择性低密度脂蛋白（LDL）单采术常用于治疗家族性高胆固醇血症（FH）患者。chemerin是一种脂肪因子，在脂质代谢调节中可能发挥作用。
在我们的初步研究中，我们使用脂蛋白直接吸附技术（DALI），通过酶联免疫吸附测定法测量了6例严重杂合子FH患者在首次LDL单采治疗前后的血清chemerin水平。
首次治疗使血清chemerin水平平均降低了27.26%。在随访一名患者时，12个月的定期LDL单采导致其血清chemerin水平持续降低。通过凝胶电泳（Lipoprint）测量的脂蛋白亚组分变化与chemerin水平降低相关。此外，我们洗脱并测量了与DALI柱结合的chemerin。
我们得出结论，LDL单采通过将chemerin蛋白结合到柱上，降低了其循环水平，从而改善了脂蛋白亚组分模式。
