Department of Immunology, Herbert Wertheim College of Medicine, Florida International University, Miami, FL-33199, USA.
Institute of Neuro-Immune Pharmacology, Herbert Wertheim College of Medicine, Florida International University, Miami, FL-33199, USA.
Sci Rep. 2016 Oct 19;6:34862. doi: 10.1038/srep34862.
Interplay between lncRNAs and mRNAs is rapidly emerging as a key epigenetic mechanism in controlling various cell functions. HIV can actively infect and/or can persist latently for years by manipulating host epigenetics; however, its molecular essence remains undiscovered in entirety. Here for the first time, we delineate the influence of HIV on global lncRNAs expression in monocytic cells lines. Our analysis revealed the expression modulation of nearly 1060 such lncRNAs which are associated with differentially expressed mRNAs in active and latent infection. This suggests a greater role of lncRNAs in regulating transcriptional and post-transcriptional gene expression during HIV infection. The differentially expressed mRNAs were involved in several different biological pathways where immunological networks were most enriched. Importantly, we discovered that HIV induces expression reversal of more than 150 lncRNAs between its active and latent infection. Also, hundreds of unique lncRNAs were identified in both infection conditions. The pathology specific "gene-expression reversal" and "on-and-off" switching of lncRNAs and associated mRNAs may lead to establish the relationship between active and HIV infection.
lncRNAs 和 mRNAs 之间的相互作用迅速成为控制各种细胞功能的关键表观遗传机制。HIV 可以通过操纵宿主表观遗传学来积极感染和/或潜伏多年；然而，其分子本质尚未完全发现。在这里，我们首次描述了 HIV 对单核细胞系中全局 lncRNAs 表达的影响。我们的分析揭示了近 1060 种 lncRNAs 的表达调控，这些 lncRNAs 与活跃和潜伏感染中差异表达的 mRNAs 相关。这表明 lncRNAs 在 HIV 感染过程中在调节转录和转录后基因表达方面发挥着更大的作用。差异表达的 mRNAs 参与了几个不同的生物学途径，其中免疫网络最为丰富。重要的是，我们发现 HIV 在其活跃和潜伏感染之间诱导了超过 150 个 lncRNAs 的表达逆转。此外，在两种感染情况下还鉴定了数百个独特的 lncRNAs。lncRNAs 和相关 mRNAs 的特定病理“基因表达逆转”和“开-关”切换可能导致建立活跃和 HIV 感染之间的关系。
