Department of Energy Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin-Madison, Madison, WI 53726, USA.; Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
Department of Microbiology and Plant Biology, The University of Oklahoma, Norman, OK 73019, USA.
Sci Adv. 2016 Oct 14;2(10):e1600393. doi: 10.1126/sciadv.1600393. eCollection 2016 Oct.
Angiosperms represent most of the terrestrial plants and are the primary research focus for the conversion of biomass to liquid fuels and coproducts. Lignin limits our access to fibers and represents a large fraction of the chemical energy stored in plant cell walls. Recently, the incorporation of monolignol ferulates into lignin polymers was accomplished via the engineering of an exotic transferase into commercially relevant poplar. We report that various angiosperm species might have convergently evolved to natively produce lignins that incorporate monolignol ferulate conjugates. We show that this activity may be accomplished by a BAHD feruloyl-coenzyme A monolignol transferase, FMT1 (AT5), in rice and its orthologs in other monocots.
被子植物代表了大部分陆生植物，也是将生物质转化为液体燃料和联产产品的主要研究对象。木质素限制了我们对纤维的利用，并且代表了植物细胞壁中储存的大量化学能。最近，通过将一种外来转移酶工程改造到商业上相关的杨树中，实现了将松柏醇阿魏酸酯单体掺入木质素聚合物中。我们报告说，各种被子植物物种可能已经趋同进化，从而天然产生含有松柏醇阿魏酸酯共轭物的木质素。我们表明，这种活性可以通过水稻中的 BAHD 阿魏酰辅酶 A 松柏醇转移酶 FMT1（AT5）及其在其他单子叶植物中的同源物来完成。
Zotero 插件
