Lin En-Ping, Chiu Tai-Chia, Hsieh Ming-Mu
Department of Chemistry, National Kaohsiung Normal University, Taiwan.
Department of Applied Science, National Taitung University, Taitung, Taiwan.
J Sep Sci. 2016 Dec;39(24):4841-4850. doi: 10.1002/jssc.201600952. Epub 2016 Nov 14.
Dispersive liquid-liquid microextraction was combined with acetonitrile stacking in capillary electrophoresis for the identification of three selective serotonin reuptake inhibitors (citalopram, fluoxetine, and fluvoxamine) in human fluids such as urine and plasma. Parameters that affect the extraction and stacking efficiency, such as the type and volume of the extraction and disperser solvent, extraction time, salt addition for dispersive liquid-liquid microextraction, and sample matrices, pH, and concentration of the separation buffer for stacking, were investigated and optimized. Under optimum conditions, the enrichment factors were in the range of 1195-1441. Limits of detection ranged from 1.4 to 1.7 nM for the target analytes. Calibration graphs displayed satisfied linearity with R greater than or equal to 0.9978, and relative standard deviations of the peak area analysis were in the range of 2.9-5.0% (n = 3). The recoveries of all tricyclic antidepressant drugs from urine and plasma were in the range of 77-117 and 79-106%, respectively. The findings of this study show that dispersive liquid-liquid microextraction acetonitrile-stacking capillary electrophoresis is a rapid and convenient method for identifying tricyclic antidepressant drugs in urine and plasma.
分散液液微萃取与毛细管电泳中的乙腈堆积相结合，用于鉴定人尿液和血浆等体液中的三种选择性5-羟色胺再摄取抑制剂（西酞普兰、氟西汀和氟伏沙明）。研究并优化了影响萃取和堆积效率的参数，如萃取溶剂和分散剂溶剂的类型及体积、萃取时间、分散液液微萃取中的加盐量、样品基质、堆积时分离缓冲液的pH值和浓度。在最佳条件下，富集因子在1195 - 1441范围内。目标分析物的检测限为1.4至1.7 nM。校准曲线显示出良好的线性，R大于或等于0.9978，峰面积分析的相对标准偏差在2.9 - 5.0%范围内（n = 3）。所有三环类抗抑郁药在尿液和血浆中的回收率分别在77 - 117%和79 - 106%范围内。本研究结果表明，分散液液微萃取乙腈堆积毛细管电泳是一种快速便捷的方法，可用于鉴定尿液和血浆中的三环类抗抑郁药。
