Naeemipour M, Hashemitabar Gh R, Dastjerdi K, Mojaver M Jamshidian, Mohammadi H R
Pol J Vet Sci. 2016 Sep 1;19(3):573-580. doi: 10.1515/pjvs-2016-0072.
From economical point of view, Dicrocoelium Dendriticum (D. dendriticum) causes a lot of damages to the livestock industry annually. So, the rapid diagnosis of infection is very important. The diagnosis is based on egg per count of feces (EPG) test because detection according to clinical symptoms is difficult. Since EPG is not accurate and sensitive, the serological methods become important for the diagnosis of this parasite as they are more accurate in comparison to EPG test and they are able to diagnose infection in a short time. In this study, somatic and Excretory-secretory antigens (EsAg) were isolated. The ELISA test was set up according to positive and negative sera and the results which were obtained compared to those obtained by the EPG test. The prevalence of infection in 550 samples by ELISA and EPG methods were 56% and 7% respectively, which shows the significant difference between these methods in examining the rate of infection. Based on the results, the specificity and sensitivity in ELISA test were 95% and 94%, respectively. The results showed that the ELISA is a more reliable test in comparison to EPG test for the rapid diagnosis of D. dendriticum infection.
从经济角度来看，枝双腔吸虫每年给畜牧业造成巨大损失。因此，快速诊断感染情况非常重要。诊断基于粪便虫卵计数（EPG）检测，因为根据临床症状进行检测很困难。由于EPG检测不够准确和灵敏，血清学方法对于该寄生虫的诊断变得很重要，因为与EPG检测相比，它们更准确，并且能够在短时间内诊断感染。在本研究中，分离出了虫体抗原和排泄分泌抗原（EsAg）。根据阳性和阴性血清建立了ELISA检测，并将所得结果与EPG检测结果进行比较。用ELISA法和EPG法检测550份样本的感染率分别为56%和7%，这表明这两种方法在检测感染率方面存在显著差异。基于这些结果，ELISA检测的特异性和敏感性分别为95%和94%。结果表明，与EPG检测相比，ELISA在枝双腔吸虫感染的快速诊断中是一种更可靠的检测方法。
