Rehbein S, Kokott S, Lindner T
Merial GmbH, Kathrinenhof Research Center, Rohrdorf, Germany.
Zentralbl Veterinarmed A. 1999 Apr;46(3):133-9. doi: 10.1046/j.1439-0442.1999.00188.x.
To evaluate the accuracy of quantitative techniques for the enumeration of Dicrocoelium dendriticum eggs in faeces, eggs were added to fluke free sheep faeces to achieve densities of 25, 50, 100, 200, 400 or 800 eggs per gram (epg) (5 replicates per density). The faecal egg counts were determined by a modified McMaster method (WETZEL, 1951) utilizing solutions of ZnSO4 (sp. g. 1.3 and 1.45), K2CO3 (sp. g. 1.45) and HgI2/KI (sp. g. 1.44) for flotation or by a sedimentation technique (4 sedimentation processes of 45 min each). The influence of flotation time on egg counts was assessed by counting the eggs 3-5, 15-20, 30-35, 45-50 and 60-65 min after loading the McMaster chamber. The results were expressed as percentage rate of recovery. Significant best rate of recovery, 91.2 +/- 9.4%, was achieved using HgI2/KI solution for flotation and there was no significant influence of flotation time on the egg count. Utilizing ZnSO4 solutions and K2CO3 solution for flotation the rates of recovery for Dicrocoelium eggs were 9.0 +/- 7.1%, 26.7 +/- 24.9% and 13.0 +/- 11.6%, respectively, and a flotation time of more than 3-5 min did significantly increase the number of floated eggs. The rate of recovery for Dicrocoelium eggs using the sedimentation technique was 41.2 +/- 1.5%.
为评估粪便中枝双腔吸虫虫卵计数定量技术的准确性，将虫卵添加到无吸虫的绵羊粪便中，使其密度达到每克25、50、100、200、400或800个虫卵（epg）（每个密度5个重复）。采用改良的麦克马斯特法（韦策尔，1951年），利用硫酸锌溶液（比重1.3和1.45）、碳酸钾溶液（比重1.45）和碘化汞/碘化钾溶液（比重1.44）进行浮选来测定粪便虫卵计数，或采用沉淀技术（每次沉淀45分钟，共4次沉淀过程）。通过在加载麦克马斯特计数室后3 - 5、15 - 20、30 - 35、45 - 50和60 - 65分钟对虫卵进行计数，评估浮选时间对虫卵计数的影响。结果以回收率百分比表示。使用碘化汞/碘化钾溶液进行浮选时，回收率最高，为91.2±9.4%，且浮选时间对虫卵计数无显著影响。使用硫酸锌溶液和碳酸钾溶液进行浮选时，枝双腔吸虫虫卵的回收率分别为9.0±7.1%、26.7±24.9%和13.0±11.6%，浮选时间超过3 - 5分钟会显著增加浮起虫卵的数量。采用沉淀技术时，枝双腔吸虫虫卵的回收率为41.2±1.5%。
