针对甘露三糖二酸基团[α-D-吡喃半乳糖-(1→6)-α-D-吡喃半乳糖-(1→6)-D-葡萄糖酸]产生的抗血清的制备及特性研究

Production and characterization of antisera raised to the manninotrionate group [alpha-D-Galp-(1----6)-alpha-D-Galp-(1----6)-D-gluconate].

作者信息

Shibata S, Plessas N, Goldstein I J

机构信息

Department of Biological Chemistry, University of Michigan, Ann Arbor 48109-0606.

出版信息

Carbohydr Res. 1989 Jun 15;189:301-7. doi: 10.1016/0008-6215(89)84106-0.

DOI:10.1016/0008-6215(89)84106-0

PMID:2776137

Abstract

Manninotrionate [alpha-D-Galp-(1----6)-alpha-D-Galp-(1----6)-D-gluconate] was coupled to bovine serum albumin (BSA), and antisera were raised in rabbits to this carbohydrate-protein conjugate. The antisera were studied by quantitative precipitation and hapten inhibition. Antisera against the manninotrionate-BSA conjugate showed the greatest reactivity with the homologous manninotrionate-BSA antigen, but also cross-reacted extensively with a manninotrionate-ovalbumin conjugate. The antisera gave weak precipitin reactions with beef-lung galactan, guaran, gum arabic, and larch arabinogalactan. Quantitative hapten-inhibition studies with a series of galactosyloligosaccharides indicated that the antisera possess a high degree of specificity toward terminal alpha-D-Galp-(1----6)-alpha-D-Galp units. The antisera did not agglutinate Ehrlich ascites tumor-cells, which have been demonstrated to contain cell-surface glycoproteins having alpha-D-galactosyl end-groups.

摘要

甘露三糖二酸酯[α-D-吡喃半乳糖-(1→6)-α-D-吡喃半乳糖-(1→6)-D-葡萄糖酸]与牛血清白蛋白（BSA）偶联，然后用这种碳水化合物-蛋白质偶联物在兔体内制备抗血清。通过定量沉淀和半抗原抑制研究这些抗血清。抗甘露三糖二酸酯-BSA偶联物的抗血清与同源的甘露三糖二酸酯-BSA抗原反应性最强，但也与甘露三糖二酸酯-卵清蛋白偶联物发生广泛的交叉反应。这些抗血清与牛肺半乳聚糖、瓜尔豆胶、阿拉伯胶和落叶松阿拉伯半乳聚糖产生微弱的沉淀反应。用一系列半乳糖基寡糖进行的定量半抗原抑制研究表明，这些抗血清对末端α-D-吡喃半乳糖-(1→6)-α-D-吡喃半乳糖单元具有高度特异性。这些抗血清不会凝集艾氏腹水瘤细胞，已证明该细胞含有具有α-D-半乳糖基末端基团的细胞表面糖蛋白。