Correia Marcelo, Sousa Maria I, Rodrigues Ana S, Perestrelo Tânia, Pereira Sandro L, Ribeiro Marcelo F, Ramalho-Santos João
Ph.D. Programme in Experimental Biology and Biomedicine (PDBEB), CNC - Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal; Institute for Interdisciplinary Research (IIIUC), University of Coimbra, Coimbra, Portugal; Biology of Reproduction and Stem Cell Group, CNC - Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.
Biology of Reproduction and Stem Cell Group, CNC - Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal; Department of Life Sciences, University of Coimbra, Coimbra, Portugal.
Data Brief. 2016 Apr 4;7:1190-1195. doi: 10.1016/j.dib.2016.03.098. eCollection 2016 Jun.
The use of new compounds as dietary supplements is increasing, but little is known in terms of possible consequences of their use. Pluripotent stem cells are a promising research tool for citotoxicological research for evaluation of proliferation, cell death, pluripotency and differentiation. Using the mouse embryonic stem cell (mESC) model, we present data on three different compounds that have been proposed as new potential supplements for co-adjuvant disease treatments: kaempferol, berberine and Tauroursodeoxycholic acid (TUDCA). Cell number and viability were monitored following treatment with increased concentrations of each drug in pluripotent culture conditions.
新型化合物作为膳食补充剂的使用正在增加,但对于其使用可能产生的后果却知之甚少。多能干细胞是细胞毒理学研究中用于评估增殖、细胞死亡、多能性和分化的一种很有前景的研究工具。利用小鼠胚胎干细胞(mESC)模型,我们展示了关于三种不同化合物的数据,这三种化合物已被提议作为辅助疾病治疗的新型潜在补充剂:山奈酚、黄连素和牛磺熊去氧胆酸(TUDCA)。在多能培养条件下,用每种药物的浓度递增处理后,监测细胞数量和活力。