用于在Biacore中对GRP78进行定向固定的肽结合测定的数据。

Data for peptide-binding assay with oriented immobilization of GRP78 in Biacore.

作者信息

Wang Sheng-Hung, Yu John

机构信息

Institute of Stem Cell and Translational Cancer Research, Chang Gung Memorial Hospital at Linkou, and Chang Gung University, Taoyuan 333, Taiwan.

出版信息

Data Brief. 2016 May 4;7:1696-1699. doi: 10.1016/j.dib.2016.04.064. eCollection 2016 Jun.

DOI:10.1016/j.dib.2016.04.064

PMID:27761511

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5063806/

Abstract

To develop peptide-conjugated liposomes for cancer imaging and therapy, the label-free surface plasmon resonance (SPR) biosensor (Biacore™) is a practical and also preferred strategy to examine protein-peptide interaction. A new Biacore protocol with "oriented immobilization" for peptide-binding assay, which overcomes the drawbacks of conventional protocols, was presented in this data article. These results were complementary to the research article Wang at al., [1], which reported a series of new cancer-targeting peptides found with HotLig software (Wang et al., 2013) [2], and this newly developed Biacore protocol.

摘要

为了开发用于癌症成像和治疗的肽缀合脂质体，无标记表面等离子体共振（SPR）生物传感器（Biacore™）是一种用于检测蛋白质 - 肽相互作用的实用且优选的策略。本文报道了一种用于肽结合测定的具有“定向固定化”的新型Biacore方案，该方案克服了传统方案的缺点。这些结果与研究文章Wang等人[1]互补，该文章报道了通过HotLig软件（Wang等人，2013年）[2]发现的一系列新的癌症靶向肽，以及这种新开发的Biacore方案。