Takagawa K, Hirosawa K
Department of Fine Morphology, University of Tokyo, Japan.
Cell Struct Funct. 1989 Jun;14(3):353-62. doi: 10.1247/csf.14.353.
Cultured fibroblasts of adult rats were used to determine whether they could take in retinol administered to the culture medium at physiological concentration. After the administration of retinol, cells were observed with a phase-contrast fluorescence light microscope (LM) and a transmission electron microscope (TEM). Retinol and retinyl fatty acyl esters (RFAE) stored in the cells were analyzed with high-performance liquid chromatography (HPLC). It was revealed that these fibroblasts could take in retinol in the medium at a concentration of 1 x 10(-7) M and store it in lipid droplets in the cytoplasm as retinyl palmitate and other RFAE.
成年大鼠的培养成纤维细胞被用于确定它们是否能够摄取以生理浓度添加到培养基中的视黄醇。在添加视黄醇后，使用相差荧光显微镜（LM）和透射电子显微镜（TEM）观察细胞。用高效液相色谱法（HPLC）分析细胞中储存的视黄醇和视黄基脂肪酰酯（RFAE）。结果显示，这些成纤维细胞能够摄取浓度为1×10（-7）M的培养基中的视黄醇，并将其作为棕榈酸视黄酯和其他RFAE储存在细胞质的脂滴中。
