Kumorek M, Kubies D, Riedel T
Department of Bioactive Polymers, Institute of Macromolecular Chemistry of the Czech Academy of Sciences, Prague, Czech Republic.
Physiol Res. 2016 Oct 20;65(Suppl 2):S253-S261. doi: 10.33549/physiolres.933427.
Understanding the behavior of single proteins at the polyelectrolyte multilayer film/solution interface is of prime importance for the designing of bio-functionalized surface coatings. In the present paper, we study the adsorption of the model proteins, albumin and lysozyme, as well as basic fibroblast growth factor (FGF-2) on a polysaccharide multilayer film composed of quaternized chitosan and heparin. Several analytical methods were used to describe the formation of the polysaccharide film and its interactions with the proteins. Both albumin and lysozyme adsorbed on quaternized chitosan/heparin films, however this process strongly depended on the terminating polysaccharide. Protein adsorption was driven mainly by electrostatic interactions between protein and the terminal layer of the film. The effective binding of FGF-2 by the heparin-terminated film suggested that other interactions could also contribute to the adsorption process. We believe that this FGF-2-presenting polysaccharide film may serve as a biofunctional surface coating for biologically-related applications.
了解单蛋白在聚电解质多层膜/溶液界面的行为对于设计生物功能化表面涂层至关重要。在本文中，我们研究了模型蛋白白蛋白、溶菌酶以及碱性成纤维细胞生长因子（FGF-2）在由季铵化壳聚糖和肝素组成的多糖多层膜上的吸附情况。使用了多种分析方法来描述多糖膜的形成及其与蛋白质的相互作用。白蛋白和溶菌酶都吸附在季铵化壳聚糖/肝素膜上，然而这个过程强烈依赖于末端多糖。蛋白质吸附主要由蛋白质与膜末端层之间的静电相互作用驱动。肝素末端膜对FGF-2的有效结合表明其他相互作用也可能对吸附过程有贡献。我们认为这种呈现FGF-2的多糖膜可作为生物相关应用的生物功能表面涂层。
