Barthe C, Carrere J, Figarella C, Guy-Crotte O
Groupe de Recherche sur les glandes exocrines, Marseille, France.
Clin Chem. 1989 Sep;35(9):1901-5.
"Cystic fibrosis protein" (CFP), a minor serum protein marker of the cystic fibrosis allele, was isolated from serum from patients with cystic fibrosis by use of the "FPLC" high-resolution chromatography system and preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. CFP currently is characterized by its isoelectric point (8.4) on isoelectric focusing. However, after the first purification steps, we identified the protein, which was present in a very low concentration, by the immunosorbent assay of Hayward et al. (J Immunol Methods 1986;91:117-22), by virtue of its immunological relationship with the "CF antigen," a protein characterized in granulocytes by these same authors [Nature (London) 1985;315:513-5]. CFP, a protein of low molecular mass, about 14 kDa, appears to be strongly associated with IgG in serum. Using the same procedure with control serum permits us to assume that CFP normally is present in serum in trace amounts.
“囊性纤维化蛋白”（CFP）是囊性纤维化等位基因的一种次要血清蛋白标志物，通过使用“快速蛋白质液相色谱”（FPLC）高分辨率色谱系统和制备型十二烷基硫酸钠-聚丙烯酰胺凝胶电泳，从囊性纤维化患者的血清中分离得到。目前，CFP通过等电聚焦时的等电点（8.4）来表征。然而，在最初的纯化步骤之后，我们借助Hayward等人的免疫吸附测定法（《免疫学方法杂志》1986年；91：117 - 22），凭借其与“CF抗原”的免疫关系鉴定出了这种浓度极低的蛋白质。这些作者在粒细胞中鉴定出了“CF抗原”这种蛋白质（《自然》（伦敦）1985年；315：513 - 5）。CFP是一种低分子量蛋白质，约14 kDa，在血清中似乎与IgG紧密相关。对对照血清采用相同程序使我们能够假定，CFP在正常血清中以痕量存在。
