Šuleková Monika, Hudák Alexander, Smrčová Miroslava
Department of Chemistry, Biochemistry and Biophysics, Institute of Pharmaceutical Chemistry, The University of Veterinary Medicine and Pharmacy in Košice, Košice 04181, Slovakia.
Molecules. 2016 Oct 17;21(10):1368. doi: 10.3390/molecules21101368.
Reversed-phase high performance liquid chromatography (RP-HPLC) for the determination of five synthetic food dyes (Quinoline Yellow E104, Sunset Yellow E110, Ponceau 4R E124, Tartrazine E102 and Carmine E120) in vitamins was used. The dyes were analyzed within 10 min using a column with stationary phase C 18 (250 mm × 4.6 mm, 5 μm) at 40 °C with isocratic elution, and the mobile phase contained acetonitrile and a mixture of CH₃COONa:CH₃OH (85:15, /) in a ratio of 10:90 (/) for yellow-colored capsules and 20:80 (/) for red-colored capsules, respectively. A diode-array detector was used to monitor the dyes between 190 and 800 nm. It was established that the analyzed samples contained synthetic dyes in a concentration range from 79.5 ± 0.01 μg/capsule of Ponceau 4R, E124 to 524 ± 0.01 μg/capsule of Tartrazine, E102. The obtained results were compared with existing acceptable daily intakes (ADIs) for individual dyes. This paper provides information about the content of dyes in samples of vitamins. This information is not generally available to consumers.
采用反相高效液相色谱法（RP-HPLC）测定维生素中的五种合成食用色素（喹啉黄E104、日落黄E110、胭脂红4R E124、柠檬黄E102和胭脂红E120）。使用C18固定相柱（250 mm×4.6 mm，5μm）在40℃下等度洗脱，10分钟内对色素进行分析，流动相分别为乙腈和CH₃COONa:CH₃OH（85:15，/）的混合物，黄色胶囊的比例为10:90（/），红色胶囊的比例为20:80（/）。使用二极管阵列检测器在190至800 nm之间监测色素。结果表明，分析的样品中合成色素的浓度范围为：胭脂红4R（E124）79.5±0.01μg/胶囊至柠檬黄（E102）524±0.01μg/胶囊。将所得结果与各色素现有的每日允许摄入量（ADI）进行比较。本文提供了维生素样品中色素含量的信息。消费者通常无法获取该信息。
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