Martin Frédéric, Oberson Jean-Marie, Meschiari Marco, Munari Caroline
Nestlé Research Center, Nestec Ltd., Vers-Chez-les Blanc, 1000 Lausanne 26, Switzerland.
Nestlé Research Center, Nestec Ltd., Vers-Chez-les Blanc, 1000 Lausanne 26, Switzerland.
Food Chem. 2016 Apr 15;197 Pt B:1249-55. doi: 10.1016/j.foodchem.2015.11.067. Epub 2015 Nov 18.
A multi-residue method based on two different extraction procedures was developed and compared with liquid chromatography electrospray ionization tandem mass spectrometry analysis of eighteen water-soluble artificial colours including Tartrazine (E102), Chrysoine (E103), Quinoline Yellow (E104), Yellow 2G (E107), Sunset Yellow (E110), Azorubine (E122), Amaranth (E123), Ponceau 4R (E124), Erythrosine (E127), Red 2G (E128), Allura Red (E129), Patent Blue V (E131), Indigo Carmine (E132), Brilliant Blue (E133), Green S (E142), Fast Green (E143), Brilliant Black (E151), and Black 7984 (E152) in sugar and gummy confectionary, ice-cream, and chocolate sweets. Sample preparation included SPE clean-up and liquid-liquid extraction for ice-cream and chocolate sweets. Accuracy was evaluated by recovery experiments. Correlation between response and concentration was obtained with R(2)>0.98 for all but six colours. Limits of quantification were within the 10-50 μg/kg range for E129; 20-200 μg/kg for E152; 10-250 μg/kg for E103; 10-500 μg/kg for E102, E104, E107, E110, E122, E123, E124, E127, E128, E131, E133; 20-800 μg/kg for E132, 142, 151; and 10-1000 μg/kg for E143. CV for repeatability ranged from 4.0% to 51.0%, while the CV for intermediate reproducibility ranged from 5.8% to 41.4%. Finally, recoveries varied from 84.3% to 166.0%. Together, these demonstrate that the method has been validated for complex matrices and is, thus, fit-for-purpose.
开发了一种基于两种不同提取程序的多残留方法，并将其与液相色谱-电喷雾电离串联质谱分析法进行比较，用于分析糖果、冰淇淋和巧克力糖果中的18种水溶性人工合成色素，包括柠檬黄（E102）、酸性金黄（E103）、喹啉黄（E104）、日落黄（E107）、亮蓝（E110）、偶氮玉红（E122）、苋菜红（E123）、丽春红4R（E124）、赤藓红（E127）、诱惑红（E128）、专利蓝V（E131）、靛蓝胭脂红（E132）、亮蓝（E133）、绿色S（E142）、亮绿（E143）、亮黑（E151）和黑色7984（E152）。样品制备包括固相萃取净化以及对冰淇淋和巧克力糖果进行液液萃取。通过回收率实验评估准确度。除六种色素外，所有色素的响应与浓度之间的相关性R²>0.98。诱惑红（E129）的定量限在10 - 50 μg/kg范围内；黑色7984（E152）为20 - 200 μg/kg；酸性金黄（E103）为10 - 250 μg/kg；柠檬黄（E102）、喹啉黄（E104）、日落黄（E107）、亮蓝（E110）、偶氮玉红（E122）、苋菜红（E123）、丽春红4R（E124）、赤藓红（E127）、诱惑红（E128）、专利蓝V（E131）、亮蓝（E133）为10 - 500 μg/kg；靛蓝胭脂红（E132）、绿色S（E142）、亮黑（E151）为20 - 800 μg/kg；亮绿（E143）为10 - 1000 μg/kg。重复性的变异系数范围为4.0%至51.0%，而中间精密度的变异系数范围为5.8%至41.4%。最后，回收率在84.3%至166.0%之间。这些结果共同表明该方法已针对复杂基质进行了验证，因此适用于目标用途。
