Shen Zhengyu, Zhang Chenping, Zhou Zengtong, Liu Wei, Shi Linjun, Shen Xuemin
Department of Dermatology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Department of Oral and Maxillofacial-Head and Neck Oncology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Oral Surg Oral Med Oral Pathol Oral Radiol. 2016 Nov;122(5):619-624.e1. doi: 10.1016/j.oooo.2016.07.005. Epub 2016 Jul 20.
To investigate the potential role of interleukin (IL)-17A and its targeting microRNAs (miRNAs) in oral lichen planus (OLP).
We compared the IL-17A expression levels in biopsies from patients with OLP (n = 50) against normal controls (n = 19) by using reverse transcription quantitative polymerase chain reaction (RT-qPCR) and Western blot analyses, identified the targeting miRNAs of IL-17A, and examined the miRNA expression levels in OLP.
IL-17A expression was significantly increased in patients with OLP compared with controls. The dual-luciferase reporter assay system demonstrated that miR-635 and miR-578 were the target miRNAs of IL-17A in human embryonic kidney 293 cells, which was consistent with predictions from bioinformatics software analyses. In contrast with IL-17A, the expression levels of miR-635 and miR-578 in OLP were significantly decreased compared with controls.
This pilot study found that low levels of miR-635 and miR-578 expression were associated with high expression of IL-17A, suggesting that IL-17A and its targeting miRNAs contribute to the pathogenesis of OLP.
探讨白细胞介素（IL）-17A及其靶向微小RNA（miRNA）在口腔扁平苔藓（OLP）中的潜在作用。
我们通过逆转录定量聚合酶链反应（RT-qPCR）和蛋白质免疫印迹分析，比较了OLP患者（n = 50）与正常对照（n = 19）活检组织中IL-17A的表达水平，鉴定了IL-17A的靶向miRNA，并检测了OLP中miRNA的表达水平。
与对照组相比，OLP患者中IL-17A表达显著增加。双荧光素酶报告基因检测系统表明，miR-635和miR-578是人类胚胎肾293细胞中IL-17A的靶向miRNA，这与生物信息学软件分析的预测结果一致。与IL-17A相反，OLP中miR-635和miR-578的表达水平与对照组相比显著降低。
这项初步研究发现，miR-635和miR-578的低表达水平与IL-17A的高表达相关，提示IL-17A及其靶向miRNA在OLP发病机制中起作用。
