Dubelaar G B, Groenewegen A C, Stokdijk W, van den Engh G J, Visser J W
Radiobiological Institute TNO, Rijswijk, The Netherlands.
Cytometry. 1989 Sep;10(5):529-39. doi: 10.1002/cyto.990100508.
An analysing flow cytometer, the optical plankton analyser (OPA), is presented. The instrument is designed for phytoplankton analysis, having a sensitivity comparable with commercially available flow cytometers, but a significantly extended particle size range. Particles of 500 microns in width and over 1,000 microns in length can be analysed. Sample flow rates of up to 55 microliters/s can be used. Also, the dynamic range of the instrument is significantly increased for particles larger than about 5 microns. The optics, hydraulics, and electronics of the instrument are described, including the best form for a low fluid shear cuvette. The new pulse quantification technique we call digital integration is presented. This technique is essential for the instrument to handle both short and very long particles with a large dynamic range. Test measurements demonstrating particle size range and dynamic range are presented. Dynamic ranges of 10,000 and 100,000 were typically observed, measuring field samples with Microcystis aeruginosa colonies, whereas one sample showed a dynamic range of 10(6). A simple method for interpretation of time of flight (TOF) data in terms of particle morphology is presented. The specifications of the instrument are given.
介绍了一种分析型流式细胞仪——光学浮游生物分析仪（OPA）。该仪器专为浮游植物分析而设计，其灵敏度与市售流式细胞仪相当，但粒径范围显著扩大。宽度达500微米、长度超过1000微米的颗粒均可进行分析。可使用高达55微升/秒的样品流速。此外，对于大于约5微米的颗粒，仪器的动态范围显著增加。描述了该仪器的光学、液压和电子系统，包括低流体剪切比色皿的最佳形式。介绍了我们称之为数字积分的新脉冲量化技术。该技术对于仪器处理具有大动态范围的短颗粒和非常长的颗粒至关重要。给出了证明粒径范围和动态范围的测试测量结果。在用铜绿微囊藻菌落测量现场样品时，通常观察到的动态范围为10000和100000，而一个样品的动态范围为10⁶。提出了一种根据颗粒形态解释飞行时间（TOF）数据的简单方法。给出了仪器的规格。
