Sieracki M E, Viles C L, Webb K L
School of Marine Science, College of William and Mary, Virginia Institute of Marine Science, Gloucester Point 23062.
Cytometry. 1989 Sep;10(5):551-7. doi: 10.1002/cyto.990100510.
This paper describes an algorithm for calculating the biovolume of cells with simple shapes, such as bacteria, flagellates, and simple ciliates, from a 2-dimensional digital image. The method can be adapted to any image analysis system which allows access to the binary cell image--(i.e., the pixels, or (x,y) points, composing the cell. The cell image is rotated to a standard orientation (horizontal), and a solid of revolution is calculated by digital integration. Verification and a critical assessment of the method are presented. The algorithm accounts for irregularities in cell shape that conventional methods based on length, width, and geometrical formulas do not.
本文描述了一种用于从二维数字图像计算具有简单形状的细胞（如细菌、鞭毛虫和简单纤毛虫）生物体积的算法。该方法可适用于任何能够访问二进制细胞图像的图像分析系统——（即组成细胞的像素或（x，y）点）。将细胞图像旋转到标准方向（水平），并通过数字积分计算旋转体。文中给出了该方法的验证和批判性评估。该算法考虑了基于长度、宽度和几何公式的传统方法未考虑的细胞形状不规则性。
