Barsotti P, Muda A O, Ascoli V, Casareale P, Nardi F
Dipartimento di Biopatologia Umana, Universita La Sapienza, Roma, Italia.
Diagn Cytopathol. 1989;5(2):154-61. doi: 10.1002/dc.2840050209.
The results of electron microscopic examination of cytologic specimens from six cases of mesothelioma and 10 cases of metastatic carcinoma of different origins are presented. The formation of cell clusters in malignant effusions from the two neoplasms has been thoroughly investigated: in mesotheliomas, cells had longer, more slender microvilli than in carcinomas and more abundant bundles of intermediate filaments; the central cavity often seen in the clusters frequently contained collagen and showed basement membrane production. The application of periodic acid-silver methenamine (PASM) and phosphotungstic acid (PTA) demonstrated a peculiar ultrastructural difference in cell coat staining in the two tumor types: in mesotheliomas, PTA and PASM were consistently negative along the outer surface of the cell aggregates, while carcinomas displayed a positive reaction either on the outer surface or on both inner and outer surfaces of the clusters. The diagnostic significance of the above-mentioned difference between the two neoplasms will require further investigation in a larger number of cases.
本文展示了对6例间皮瘤和10例不同起源的转移性癌的细胞学标本进行电子显微镜检查的结果。对这两种肿瘤的恶性积液中细胞团簇的形成进行了深入研究：间皮瘤细胞的微绒毛比癌细胞更长、更细，中间丝束更丰富；细胞团簇中常见的中央腔通常含有胶原蛋白，并显示有基底膜产生。高碘酸-银甲胺（PASM）和磷钨酸（PTA）的应用显示出两种肿瘤类型在细胞表面染色方面存在特殊的超微结构差异：在间皮瘤中，细胞聚集体外表面的PTA和PASM始终呈阴性，而癌在团簇的外表面或内外表面均显示阳性反应。两种肿瘤上述差异的诊断意义需要在更多病例中进一步研究。
