Swiderska Bianka, Kedracka-Krok Sylwia, Plytycz Barbara
Department of Physical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland; Laboratory of Proteomics and Mass Spectrometry, Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland.
Department of Evolutionary Immunology, Institute of Zoology, Faculty of Biology and Earth Sciences, Jagiellonian University, Kraków, Poland.
Data Brief. 2016 Sep 29;9:629-634. doi: 10.1016/j.dib.2016.09.035. eCollection 2016 Dec.
The data described are related to the article "Lysenin family proteins in earthworm coelomocytes - comparative approach" (B. Swiderska, S. Kedracka-Krok, T. Panz, A.J. Morgan, A. Falniowski, P.Grzmil, B. Plytycz, 2016) [1]. Lysenin family proteins were identified based on unique peptides sequenced by tandem mass spectrometry coupled with liquid chromatography (LC-MS/MS) in lumbricid earthworms and , the latter with or without the MUG-like fluorophore. Lysenin and lysenin-related protein 2 (LRP-2, fetidin) were identified in all 9 investigated specimens of sp. LRP-1 was identified in 5 of 6 specimens of , while LRP-3 was present in 2 of 3 investigated specimens of . Here, the detailed characteristics of identified peptides unique to the particular members of lysenin family present in each particular earthworm specimen was provided. The information concerning mass to charge ratio, retention time, modifications and score of unique peptides was given.
所描述的数据与文章《蚯蚓体腔细胞中的赖氨酸家族蛋白——比较方法》(B. 斯维德尔斯卡、S. 凯德拉克 - 克罗克、T. 潘兹、A.J. 摩根、A. 法尔尼夫斯基、P. 格日米尔、B. 普利蒂茨,2016年)[1]相关。基于通过液相色谱串联质谱法(LC-MS/MS)测序的独特肽段,在正蚓科蚯蚓中鉴定出赖氨酸家族蛋白,后者带有或不带有MUG样荧光团。在所有9个被研究的 种标本中鉴定出了赖氨酸和赖氨酸相关蛋白2(LRP - 2,蚯蚓抑肽)。在6个 种标本中的5个中鉴定出了LRP - 1,而在3个被研究的 种标本中的2个中存在LRP - 3。这里提供了每个特定蚯蚓标本中赖氨酸家族特定成员特有的已鉴定肽段的详细特征。给出了关于独特肽段的质荷比、保留时间、修饰和得分的信息。