在玻璃纸圆盘上制备的大肠杆菌粗裂解物中的体外转录。
In vitro transcription in E. coli crude lysates prepared on cellophane discs.
作者信息
Valla S, Lindqvist B H
出版信息
Nucleic Acids Res. 1978 Jul;5(7):2665-78. doi: 10.1093/nar/5.7.2665.
An in vitro RNA-synthesizing system consisting of gently lysed E. coli cells on cellophane discs is described. The system has been optimalized with respect to total RNA synthesis. Under certain standard conditions DNA dependent RNA polymerase (EC 2.7.7.6) is responsible for the majority of the RNA synthesis. The extensive rifampicin sensitivity of the synthesis indicates that most of the transcripts are initiated in vitro. The RNA synthesizing system described here has been developed with the aim of studying phage transcription in vitro. We show here that lysates of a P4 infected P2 lysogen support initiation and propagation of transcription from the P2 prophage.
描述了一种体外RNA合成系统，该系统由置于玻璃纸圆盘上的经温和裂解的大肠杆菌细胞组成。该系统已针对总RNA合成进行了优化。在某些标准条件下，依赖DNA的RNA聚合酶（EC 2.7.7.6）负责大部分RNA合成。合成对利福平的广泛敏感性表明，大多数转录本是在体外起始的。此处描述的RNA合成系统是为了研究噬菌体的体外转录而开发的。我们在此表明，P4感染的P2溶原菌的裂解物支持P2原噬菌体转录的起始和延伸。
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