Lehmann Christian, Fisher Nicholas B, Tugwell Barna, Szczesniak Anna, Kelly Mel, Zhou Juan
Department of Anesthesia, Pain Management and Perioperative Medicine, Dalhousie University, Halifax, NS, Canada.
Department of Pharmacology, Dalhousie University, Halifax, NS, Canada.
Clin Hemorheol Microcirc. 2016;64(4):655-662. doi: 10.3233/CH-168021.
Destruction of the insulin-producing beta cells in type 1 diabetes (T1D) is induced by invasion of immune cells causing pancreatic inflammation. Cannabidiol (CBD), a phytocannabinoid, derived from the plant, Cannabis sativa, was shown to lower the incidence of diabetes in non-obese diabetic (NOD) mice, an animal model of spontaneous T1D development.
The goal of this study was to investigate the impact of experimental CBD treatment on early pancreatic inflammation in T1D by intravital microscopy (IVM) in NOD mice.
Seven-week-old female NOD mice were prophylactically administered daily 5 mg/kg CBD or control vehicle i.p. five times weekly for ten weeks. Animals underwent IVM following confirmation of T1D diagnosis by blood glucose testing. Leukocyte activation and functional capillary density (FCD) were quantified via IVM.
CBD-treated NOD mice developed T1D later and showed significantly reduced leukocyte activation and increased FCD in the pancreatic microcirculation.
Experimental CBD treatment reduced markers of inflammation in the microcirculation of the pancreas studied by intravital microscopy.
1型糖尿病（T1D）中产生胰岛素的β细胞的破坏是由免疫细胞入侵导致胰腺炎症引起的。大麻二酚（CBD）是一种从植物大麻中提取的植物大麻素，已证明可降低非肥胖糖尿病（NOD）小鼠（一种自发性T1D发展的动物模型）的糖尿病发病率。
本研究的目的是通过对NOD小鼠进行活体显微镜检查（IVM），研究实验性CBD治疗对T1D早期胰腺炎症的影响。
7周龄雌性NOD小鼠预防性地每天腹腔注射5mg/kg CBD或对照载体，每周5次，持续10周。通过血糖测试确认T1D诊断后，对动物进行IVM。通过IVM对白细胞活化和功能性毛细血管密度（FCD）进行定量。
CBD治疗的NOD小鼠患T1D的时间较晚，并且胰腺微循环中的白细胞活化显著降低，FCD增加。
实验性CBD治疗降低了通过活体显微镜检查研究的胰腺微循环中的炎症标志物。
