Tu Hung-Ya, Hsu Chih-Chun, Chen Yu-Jiun, Chen Ching-Kang
Department of Ophthalmology, Baylor College of Medicine.
Department of Ophthalmology, Baylor College of Medicine; Department of Neuroscience, Baylor College of Medicine.
J Vis Exp. 2016 Oct 13(116):54608. doi: 10.3791/54608.
The mammalian retina is a layered tissue composed of multiple neuronal types. To understand how visual signals are processed within its intricate synaptic network, electrophysiological recordings are frequently used to study connections among individual neurons. We have optimized a flat-mount preparation for patch clamp recording of genetically marked neurons in both GCL (ganglion cell layer) and INL (inner nuclear layer) of mouse retinas. Recording INL neurons in flat-mounts is favored over slices because both vertical and lateral connections are preserved in the former configuration, allowing retinal circuits with large lateral components to be studied. We have used this procedure to compare responses of mirror-partnered neurons in retinas such as the cholinergic starburst amacrine cells (SACs).
哺乳动物的视网膜是一种由多种神经元类型组成的分层组织。为了了解视觉信号如何在其复杂的突触网络中进行处理，电生理记录经常被用于研究单个神经元之间的连接。我们优化了一种平铺标本制备方法，用于对小鼠视网膜神经节细胞层（GCL）和内核层（INL）中基因标记的神经元进行膜片钳记录。与切片相比，平铺标本记录INL神经元更具优势，因为在前者的配置中，垂直和横向连接都得以保留，从而能够研究具有大的横向成分的视网膜回路。我们已经使用这个程序来比较视网膜中镜像配对神经元的反应，比如胆碱能无长突星形细胞（SACs）。
