Saigusa M
Biol Bull. 1995 Oct;189(2):175-184. doi: 10.2307/1542468.
Hatch water (the filtrated medium into which zoea larvae have been released) of the estuarine terrestrial crab Sesarma haematocheir (akate-gani) contains a substance that causes premature detachment of embryos from ovigerous females. Detachment occurs when the ovigerous hairs along the female's ovigerous setae slip out of the investment coat that binds them to the embryos through stalks, or funiculi. The active factor, which I call ovigeroushair stripping substance (OHSS), is released outside of the egg capsule at the time of hatching, and is not secreted by the female. This study describes the results of a quantitative assay for measuring the activity of OHSS. Activity is measured as the percentage of hairs on a seta that can be induced to slip out of the coat without damage. Experiments with an extract of crushed embryos indicated that OHSS is present up to 2 days before hatching. Its activity was destroyed by heat and trypsin, suggesting that it is a protein. Its molecular size was estimated by gel filtration to be 15-20 kDa in S. haematocheir and 30 kDa in S. pictum. Reciprocal tests among different species indicated that OHSS occurs widely in intertidal and estuarine crabs.
河口陆生蟹红螯相手蟹（赤手蟹）的孵化水（已放入溞状幼体的过滤介质）中含有一种物质，可导致胚胎过早地从抱卵雌蟹身上脱离。当雌蟹抱卵刚毛上的抱卵毛从通过柄或细索将它们与胚胎相连的包被中滑出时，就会发生脱离。这种活性因子，我称之为抱卵毛剥离物质（OHSS），在孵化时释放到卵囊外，并非由雌蟹分泌。本研究描述了测量OHSS活性的定量测定结果。活性以刚毛上可被诱导无损滑出包被的毛发百分比来衡量。对碾碎胚胎提取物的实验表明，OHSS在孵化前2天就已存在。其活性被加热和胰蛋白酶破坏，表明它是一种蛋白质。通过凝胶过滤估计其分子大小在红螯相手蟹中为15 - 20 kDa，在图画相手蟹中为30 kDa。不同物种间的相互测试表明，OHSS广泛存在于潮间带和河口蟹类中。
