甲砜霉素治疗期间的造血作用。I. 中间细胞阶段消除过程中干细胞的刺激。

Hemopoiesis during thiamphenicol treatment. I. Stimulation of stem cells during eradication of intermediate cell stages.

作者信息

Goris H, Loeffler M, Bungart B, Schmitz S, Nijhof W

机构信息

Laboratory of Physiological Chemistry, University of Groningen, The Netherlands.

出版信息

Exp Hematol. 1989 Oct;17(9):957-61.

PMID:2776855

Abstract

Continuous treatment of C57bl/6 mice for 4 days with the cytostatic antibiotic thiamphenicol revealed a dual response of hemopoietic cells. On one hand, morphologically recognizable erythroid precursors and late progenitors (erythroid colony-forming units; CFU-E) and, to a lesser extent, granuloid precursors were found substantially reduced. On the other hand, early granuloid (granulocyte-macrophage colony-forming units; CFU-GM) and erythroid (erythroid burst-forming units; BFU-E) progenitors increased on day 3 to 220%-240% and 120%-130% of the control value, respectively. This was accompanied by a decline of the initial spleen colony-forming units (CFU-S) (day 8) pool size to approximately 60%. These patterns were similar in the bone marrow and the spleen. In addition, the tritiated thymidine kill of femoral and splenic CFU-S rose significantly (p less than 0.05) from 16% +/- 3% to 38% +/- 2% and from 3% +/- 1% to 17% +/- 2%, respectively. A sudden decline of peripheral reticulocytes between days 2 and 3 from 2.8% +/- 0.3% to 0.6% +/- 0.2% was observed, whereas the hematocrit gradually decreased from day 1 to day 4 from 45.2% +/- 0.1% to 39.3% +/- 0.3%. The white blood cells were not affected. From these results we conclude that stem cells were stimulated as a consequence of the suppression of the intermediate cell stages. As analyzed in the accompanying paper, this confirms a prediction stated by a quantitative theoretical concept of in vivo stem cell regulation.

摘要

用细胞生长抑制性抗生素甲砜霉素对C57bl/6小鼠连续治疗4天，结果显示造血细胞出现双重反应。一方面，形态上可识别的红系前体细胞和晚期祖细胞（红系集落形成单位；CFU-E），以及程度较轻的粒系前体细胞，数量大幅减少。另一方面，早期粒系（粒细胞-巨噬细胞集落形成单位；CFU-GM）和红系（红系爆式集落形成单位；BFU-E）祖细胞在第3天分别增加到对照值的220%-240%和120%-130%。与此同时，初始脾集落形成单位（CFU-S）（第8天）的库容量下降至约60%。骨髓和脾脏中的这些模式相似。此外，股骨和脾脏CFU-S的氚标记胸腺嘧啶核苷杀伤率分别从16%±3%显著上升至38%±2%（p＜＜0.05），从3%±1%上升至17%±2%。观察到外周网织红细胞在第2天至第3天之间突然从2.8%±0.3%降至0.6%±0.2%，而血细胞比容从第1天至第4天逐渐从45.2%±0.1%降至39.3%±0.3%。白细胞未受影响。从这些结果我们得出结论，由于中间细胞阶段受到抑制，干细胞受到了刺激。如随附论文中所分析的，这证实了体内干细胞调节定量理论概念所提出的一个预测。