Cdc42 通过 ERK 通路调节 LPS 诱导的原代肺微血管内皮细胞增殖。
Cdc42 regulates LPS-induced proliferation of primary pulmonary microvascular endothelial cells via ERK pathway.
机构信息
Department of Respiratory and Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Department of Histology and Embryology, School of Basic Medical Sciences Southern Medical University, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Guangzhou 510515, China.
出版信息
Microvasc Res. 2017 Jan;109:45-53. doi: 10.1016/j.mvr.2016.10.001. Epub 2016 Oct 18.
BACKGROUND
After stimulation due to injury, cell division cycle protein 42 (Cdc42) restores and enhances barrier functions by strengthening intercellular adherens junctions; however, its influence on cell proliferation after injury remains unknown.
OBJECTIVE
In this study, we sought to investigate the effect of stimulation using small doses of lipopolysaccharide (LPS) on the proliferation of pulmonary microvascular endothelial cells (PMVECs).
METHODS
We stimulated PMVECs with different doses of LPS and evaluated the effects on cell proliferation. We also constructed a primary gene-knockout cell line lacking Cdc42 to verify the role of Cdc42 in regulating the proliferation of PMVECs that were stimulated using LPS and to explore related signaling pathways.
RESULTS
Stimulating PMVECs with small doses of LPS increased proliferation. Cdc42 is involved in regulating this process, which was mediated by the extracellular regulated protein kinase (ERK) pathway.
CONCLUSIONS
Cdc42 plays a role in regulating the proliferation of PMVECs stimulated with small doses of LPS, and this regulation involves the ERK pathway.
背景
在损伤刺激后，细胞分裂周期蛋白 42（Cdc42）通过增强细胞间黏附连接来恢复和增强屏障功能；然而，其对损伤后细胞增殖的影响尚不清楚。
目的
本研究旨在探讨小剂量脂多糖（LPS）刺激对肺微血管内皮细胞（PMVECs）增殖的影响。
方法
我们用不同剂量的 LPS 刺激 PMVECs，评估其对细胞增殖的影响。我们还构建了一个缺乏 Cdc42 的原代基因敲除细胞系，以验证 Cdc42 在调节 LPS 刺激的 PMVECs 增殖中的作用，并探讨相关信号通路。
结果
小剂量 LPS 刺激 PMVECs 可促进增殖。Cdc42 参与了这一过程的调节，其机制涉及细胞外调节蛋白激酶（ERK）通路。
结论
Cdc42 在调节小剂量 LPS 刺激的 PMVECs 增殖中发挥作用，这种调节涉及 ERK 通路。
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