Bilichak Andriy, Kovalchuk Igor
Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, AB, Canada.
Department of Biological Sciences, University of Lethbridge, 4401 University Drive, Lethbridge, AB, Canada, T1K 3M4.
Methods Mol Biol. 2017;1456:225-236. doi: 10.1007/978-1-4899-7708-3_17.
Virus-induced gene silencing (VIGS) is a powerful epigenetic tool that allows in a relatively short period of time to down-regulate the expression of an endogenous gene in infected plants for either monitoring the resulting phenotype or enhancing/modifying a particular trait associated with the gene. Here, we describe the utilization of Tobacco rattle virus (TRV) as a vector for the VIGS technique in Arabidopsis plants. The unique ability of TRV to infect both somatic tissues and gametes allows deciphering the role of genes in these tissues simultaneously. As an example, we demonstrate the utilization of TRV to down-regulate the expression of AGO2 and NRPD1a genes in ovules of Arabidopsis plants in order to boost the stable transformation efficiency by floral dip.
病毒诱导的基因沉默（VIGS）是一种强大的表观遗传工具，它能在相对较短的时间内下调受感染植物中内源基因的表达，用于监测由此产生的表型或增强/修饰与该基因相关的特定性状。在此，我们描述了利用烟草脆裂病毒（TRV）作为载体在拟南芥植物中进行VIGS技术的应用。TRV感染体细胞组织和配子的独特能力使得能够同时解读基因在这些组织中的作用。例如，我们展示了利用TRV下调拟南芥植物胚珠中AGO2和NRPD1a基因的表达，以通过花浸法提高稳定转化效率。
