Department of Biochemistry, Shivaji University, Kolhapur, Maharashtra, India.
Department of Biochemistry, School of Life Sciences, North Maharashtra University, Jalgaon, Maharashtra, India.
Pest Manag Sci. 2017 Jul;73(7):1382-1390. doi: 10.1002/ps.4467. Epub 2016 Dec 26.
Identification and characterisation of plant defensive molecules enrich our resources to design crop protection strategies. In particular, plant-derived proteinaceous inhibitor(s) of insect digestive enzymes appear to be a safe, sustainable and attractive option.
A glycoprotein having non-competitive α-amylase inhibitory activity with a molecular weight of 8.3 kDa was isolated and purified from seeds of Withania somnifera α-amylase inhibitor (WSAI). Its mass spectrometry analysis revealed 59% sequence coverage with Wrightide II-type α-amylase inhibitor from Wrightia religiosa. A dose-dependent inhibition of α-amylases from Aspergillus oryzae, Bacillus subtilis, Helicoverpa armigera and Tribolium castaneum was recorded. Interestingly, WSAI did not inhibit human salivary α-amylase significantly. When adults of T. castaneum were fed with WSAI (1.6 mg g ), decrease in consumption, growth and efficiency of conversion of ingested food was evident, along with over fourfold increases in feeding deterrence index. A decline in larval residual α-amylase activity after feeding of WSAI resulted in a reduction in longevity of T. castaneum.
The study reflects the significance of WSAI in affecting the overall growth and development of T. castaneum. Pre- and post-harvest pest resistive capability makes WSAI a potential candidate for insect pest management. Further, the effectiveness of this inhibitor could be explored either in formulations or through a transgenic approach. © 2016 Society of Chemical Industry.
鉴定和描述植物防御分子可丰富我们的资源，以设计作物保护策略。特别是，来源于植物的、能抑制昆虫消化酶的蛋白抑制剂似乎是一种安全、可持续且有吸引力的选择。
从茄科植物睡茄种子中分离并纯化得到一种 8.3 kDa 的糖蛋白，该糖蛋白对 α-淀粉酶具有非竞争性抑制活性。其质谱分析显示与来自印度木蓝的 Wrightide II 型 α-淀粉酶抑制剂有 59%的序列覆盖度。结果表明，该糖蛋白能剂量依赖性地抑制来自米曲霉、枯草芽孢杆菌、棉铃虫和赤拟谷盗的 α-淀粉酶。有趣的是，WSAI 对人唾液 α-淀粉酶的抑制作用并不明显。当赤拟谷盗成虫喂食 WSAI（1.6 mg g）时，其摄食量、增长率和食物转化率均明显降低，同时摄食拒食指数增加了四倍以上。喂食 WSAI 后，幼虫残余 α-淀粉酶活性下降，导致赤拟谷盗寿命缩短。
本研究反映了 WSAI 对赤拟谷盗整体生长和发育的影响。其具有的抗虫害能力可用于作物的产前产后保护，使其成为一种潜在的虫害管理候选物。此外，还可通过制剂或转基因的方法来探索该抑制剂的有效性。 © 2016 英国化学学会
