Ay Birol, Karaoz Erdal, Kesemenli Cumhur C, Kenar Halime
Stem Cell Department, Kocaeli University, Institute of Health Sciences, Kocaeli, Turkey.
Center for Stem Cell and Gene Therapies Research and Practice, Kocaeli University, Kocaeli, Turkey.
J Biomed Mater Res A. 2017 Mar;105(3):879-890. doi: 10.1002/jbm.a.35948. Epub 2016 Dec 5.
The reconstruction of skeletal muscle tissue is currently performed by transplanting a muscle tissue graft from local or distant sites of the patient's body, but this practice leads to donor site morbidity in case of large defects. With the aim of providing an alternative treatment approach, skeletal muscle tissue formation potential of human myoblasts and human menstrual blood derived mesenchymal stem cells (hMB-MSCs) on synthetic [poly(l-lactide-co-caprolactone), 70:30] scaffolds with oriented microfibers, human muscle extracellular matrix (ECM), and their hybrids was investigated in this study. The reactive muscle ECM pieces were chemically crosslinked to the synthetic scaffolds to produce the hybrids. Cell proliferation assay WST-1, scanning electron microscopy (SEM), and immunostaining were carried out after culturing the cells on the scaffolds. The ECM and the synthetic scaffolds were effective in promoting spontaneous myotube formation from human myoblasts. Anisotropic muscle patch formation was more successful when human myoblasts were grown on the synthetic scaffolds. Nonetheless, spontaneous differentiation could not be induced in hMB-MSCs on any type of the scaffolds. Human myoblast-synthetic scaffold combination is promising as a skeletal muscle patch, and can be improved further to serve as a fast integrating functional patch by introducing vascular and neuronal networks to the structure. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 879-890, 2017.
目前，骨骼肌组织的重建是通过移植患者身体局部或远处的肌肉组织移植物来进行的，但对于大面积缺损而言，这种做法会导致供体部位出现并发症。为了提供一种替代治疗方法，本研究探讨了人成肌细胞和人月经血源性间充质干细胞（hMB-MSCs）在具有定向微纤维的合成[聚（L-丙交酯-共-己内酯），70:30]支架、人肌肉细胞外基质（ECM）及其杂化物上形成骨骼肌组织的潜力。将具有反应活性的肌肉ECM片段化学交联到合成支架上以制备杂化物。在支架上培养细胞后，进行细胞增殖测定WST-1、扫描电子显微镜（SEM）和免疫染色。ECM和合成支架在促进人成肌细胞自发形成肌管方面是有效的。当人成肌细胞在合成支架上生长时，各向异性肌肉贴片的形成更为成功。然而，在任何类型的支架上，hMB-MSCs都不能被诱导自发分化。人成肌细胞与合成支架的组合有望成为一种骨骼肌贴片，通过在结构中引入血管和神经网络，可进一步改进以作为一种快速整合的功能性贴片。©2016威利期刊公司。《生物医学材料研究杂志》A部分：105A:879 - 890，2017年。
