Kahya Mehmet Cemal, Sebik Oğuz, Türker Kemal S
Department of Biophysics, İzmir Katip Çelebi University School of Medicine, İzmir, Turkey.
Department of Physiology, Koç University School of Medicine, Istanbul, Turkey.
J Electromyogr Kinesiol. 2016 Dec;31:104-110. doi: 10.1016/j.jelekin.2016.10.002. Epub 2016 Oct 14.
Painful stimulation of the hand results in an inhibitory response in the hand muscles known as the cutaneous silent period (CSP). In this study, we employed probability- and frequency-based analysis methods to examine the CSP induced by laser stimuli. Subjects were asked to contract their first dorsal interosseous muscle so that selected motor units discharged at a rate of about 8Hz. Laser pulses were delivered to the palm of the hand, and reflex responses were recorded. The stimuli generated CSP in all test subjects. We found that the latency of the CSP evoked using laser stimulation was longer than that the previously published latency values of the CSP evoked using electrical stimulation. Using only the presently generated laser induced CSP data, the CSP duration was longer when analyzed via peristimulus frequencygram method compared to the probability-based methods such as peristimulus time histogram and surface electromyogram. In the light of the current results, we suggest that laser stimulation could be used when studying pain pathways in human subjects and the frequency-based analysis methods can be preferred because they are previously shown to be more reliable for obtaining the synaptic activity profile. These results can be used to standardize the CSP methods in basic and clinical research.
对手部进行疼痛刺激会导致手部肌肉产生一种抑制性反应，即皮肤静息期（CSP）。在本研究中，我们采用基于概率和频率的分析方法来检测激光刺激诱发的CSP。要求受试者收缩其第一背侧骨间肌，使选定的运动单位以约8Hz的频率放电。将激光脉冲施加于手掌，并记录反射反应。所有受试对象均产生了CSP。我们发现，使用激光刺激诱发的CSP的潜伏期比先前发表的使用电刺激诱发的CSP的潜伏期值更长。仅使用当前生成的激光诱发CSP数据时，与基于概率的方法（如刺激时间直方图和表面肌电图）相比，通过刺激频率图方法分析时CSP持续时间更长。根据当前结果，我们建议在研究人类受试者的疼痛通路时可使用激光刺激，并且基于频率的分析方法可能更可取，因为先前已表明它们在获取突触活动概况方面更可靠。这些结果可用于基础和临床研究中CSP方法的标准化。
