Benson-Chanda V, Su M W, Weil D, Chu M L, Ramirez F
Department of Microbiology and Immunology, State University of New York, Brooklyn 11203.
Gene. 1989 May 30;78(2):255-65. doi: 10.1016/0378-1119(89)90228-x.
We have isolated overlapping clones containing the 5'-terminal portion of the human pro-alpha 1(III) collagen gene (COL3A1). This has enabled us to extend our previous studies and thus generate a restriction map of nearly 64 kb of DNA encompassing all of COL3A1 and more than 20 kb of flanking sequences. Aside from the complete nucleotide and amino acid sequences of type-III N-pre-propeptide, this study has established the number of the corresponding exons, whose relative organization deviates from the pattern observed in the analogous regions of type-I procollagen genes, COL1A1 and COL1A2. Moreover, we have sequenced 1628 bp of the 5'-flanking region of COL3A1, from the transcription start point (tsp) to an AluI repetitive element. Pairwise comparison with the analogous segment of the mouse gene has showed 78% sequence similarity in nearly 270 bp immediately preceding the tsp and including the TATA element and a presumptive NF-1 binding site. Relatively close to the tsp, but upstream from the region of homology with the murine gene, a potential AP-1 binding site has also been identified.
我们已经分离出了包含人原α1(III)胶原基因（COL3A1）5'-末端部分的重叠克隆。这使我们能够扩展之前的研究，从而生成一个近64 kb DNA的限制性图谱，该图谱涵盖了整个COL3A1以及超过20 kb的侧翼序列。除了III型N-前原肽的完整核苷酸和氨基酸序列外，本研究还确定了相应外显子的数量，其相对组织方式与I型前胶原基因COL1A1和COL1A2类似区域中观察到的模式不同。此外，我们对COL3A1的5'-侧翼区域进行了1628 bp的测序，从转录起始点（tsp）到一个AluI重复元件。与小鼠基因的类似片段进行成对比较显示，在tsp之前紧邻的近270 bp区域，包括TATA元件和一个假定的NF-1结合位点，序列相似性为78%。在相对靠近tsp但位于与小鼠基因同源区域上游的位置，还鉴定出了一个潜在的AP-1结合位点。
