SnCoO (x=0.00, 0.01, 0.03, 0.05) nanoparticles (NPs) of average size ∼30-40nm were synthesized by co-precipitation method. The interaction of Co doped SnO NPs with human serum albumin (HSA) and their photocatalytic and antimicrobial properties were studied. The structural analysis and morphology of Co doped SnO NPs were analysed via X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), high resolution transmission electron microscopy (HRTEM) and Fourier transform infrared spectroscopy (FT-IR). Besides the structural and morphological analysis, the interaction of Co doped SnO NPs with HSA were studied by UV-vis, Circular dichroism (CD) and fluorescence spectroscopy. Fluorescence quenching results suggest that Co doped SnO NPs interact with an HSA molecule through static mechanism. CD indicates that α-helicity of HSA increases due to the interaction of Co doped SnO NPs. The photocatalytic activities of the NPs with increased doping concentration were evaluated through a degradation process in the presence of methylene-blue (MB) dye under UV light irradiation, which exhibited that the surface area of NPs with increased doping concentration plays a major role in improving the photocatalytic activity. The antimicrobial effect of undoped and Co-doped SnO NPs was determined using agar-well diffusion method and analyzed against gram-positive bacteria (Bacillus Cereus MC 2434). In our results, we have found that as the doping concentration increases into NPs, zone of inhibition increases, which could be ascribed to the production of ROS and large surface area of the NPs.