Azizah Nor, Hashim Uda, Gopinath Subash C B, Nadzirah Sharipah
Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, 01000 Kangar, Perlis, Malaysia.
Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, 01000 Kangar, Perlis, Malaysia; School of Microelectronic Engineering, Universiti Malaysia Perlis, 01000 Kangar, Perlis, Malaysia.
Int J Biol Macromol. 2017 Jan;94(Pt A):571-575. doi: 10.1016/j.ijbiomac.2016.10.060. Epub 2016 Oct 19.
Nanoparticles have been investigated as flagging tests for the sensitive DNA recognition that can be utilized as a part of field applications to defeat restrictions. Gold nanoparticles (AuNPs) have been widely utilized due to its optical property and capacity to get functionalized with a mixed bag of biomolecules. This study exhibits the utilization of AuNPs functionalized with single-stranded oligonucleotide (AuNP-oligo test) for fast the identification of Human Papillomavirus (HPV). This test is displayed on interdigitated electrode sensor and supported by colorimetric assay. DNA conjugated AuNP has optical property that can be controlled for the applications in diagnostics. With its identification abilities, this methodology incorporates minimal effort, strong reagents and basic identification of HPV.
纳米颗粒已被研究作为用于灵敏DNA识别的标记测试，可用于现场应用以克服限制。金纳米颗粒（AuNPs）因其光学性质以及能够用多种生物分子进行功能化修饰而被广泛应用。本研究展示了用单链寡核苷酸功能化的金纳米颗粒（AuNP - 寡核苷酸测试）用于快速鉴定人乳头瘤病毒（HPV）。该测试展示在叉指电极传感器上，并由比色测定法支持。DNA共轭金纳米颗粒具有可用于诊断应用的光学性质。凭借其识别能力，该方法所需工作量最少、试剂耐用且对HPV的鉴定方法简单。
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