Department of Chemistry, College of Science, Yanbian University, Yanji, Jilin, 133002, China.
Education Department of the Teachers College, Yanbian University, Yanji, Jilin, 133002, China.
Mikrochim Acta. 2018 Nov 9;185(12):537. doi: 10.1007/s00604-018-3065-2.
A colorimetric assay for human papillomavirus (HPV) DNA was developed based on the retardation of the avidin-induced aggregation of gold nanoparticles (AuNPs) by HPV DNA. Positively charged avidin acts as a coagulant for AuNP aggregation. In the presence of the target DNA, however, the aggregation of AuNPs is retarded owing to electrosteric stabilization as a result of the hybridization of the target and probe DNA. In the absence of HPV DNA, the stabilization effect caused by the biotinylated probe DNA is weak, resulting in NP aggregation and a color change from red to purple. Aggregation may be easily observed with bare eyes or spectrophotometrically at about 560 nm. The visual detection limit is 1 nM. The assay was used for the determination of HPV DNA after polymerase chain reaction (PCR) amplification without any further purification. Graphical abstract Schematic presentation of the avidin-induced aggregation of unmodified gold nanoparticles (AuNPs) which leads to a color change from red to purple. In the presence of dsDNA, however, the aggregation is remarkably retarded.
基于人乳头瘤病毒（HPV）DNA 对金纳米粒子（AuNPs）的诱导聚集的阻滞作用，开发了一种用于 HPV DNA 的比色测定法。带正电荷的亲和素作为 AuNP 聚集的凝结剂。然而，在存在靶 DNA 的情况下，由于目标和探针 DNA 的杂交导致的空间稳定作用，AuNPs 的聚集被阻滞。在没有 HPV DNA 的情况下，由于生物素化探针 DNA 引起的稳定作用较弱，导致 NP 聚集和颜色从红色变为紫色。聚集可以用肉眼或分光光度计在约 560nm 处轻松观察到。视觉检测限为 1 nM。该测定法用于聚合酶链反应（PCR）扩增后 HPV DNA 的测定，无需进一步纯化。
