Hunan Vocational Institute of Safety Technology, Changsha, 410151, China.
Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education),College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, China.
J Fluoresc. 2017 Jan;27(1):205-211. doi: 10.1007/s10895-016-1947-4. Epub 2016 Oct 22.
In this paper, we report a simple and sensitive fluorescent biosensor for the quantitative analysis of silver ions (Ag) by using NaYF:Yb, Tm upconversion nanoparticles (UCNPs). Ag could oxidize o-phenylenediamine (OPD) to the oxidized OPD (oxOPD) directly. The fluorescence of UCNPs can be significantly quenched by oxOPD through inner filter effects (IFE). Under the optimized conditions, the Ag concentration is proportional to the changes of the fluorescence intensity of UCNPs. The proposed method shows high selectivity and Ag could be quantitatively detected in the range of 0 to 0.5 mM with a low detection limit of 33 nM for Ag. The selectivity and sensitivity of the detection can also be satisfactory. More importantly, this method has potential in practical application to detect Ag in real samples without interference.
在本文中，我们报告了一种简单灵敏的荧光生物传感器，用于通过 NaYF:Yb，Tm 上转换纳米粒子 (UCNPs) 定量分析银离子 (Ag)。Ag 可以直接将邻苯二胺 (OPD) 氧化为氧化的 OPD (oxOPD)。UCNPs 的荧光可以通过内滤效应 (IFE) 被 oxOPD 显著猝灭。在优化条件下，Ag 的浓度与 UCNPs 荧光强度的变化成正比。所提出的方法具有高选择性，Ag 可以在 0 到 0.5 mM 的范围内定量检测，Ag 的检测限低至 33 nM。检测的选择性和灵敏度也令人满意。更重要的是，该方法具有在实际样品中检测 Ag 而不受干扰的潜在应用。
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