Panikkar B, Kuttan R
Indian J Biochem Biophys. 1989 Apr;26(2):126-8.
A method has been described for the detection and estimation of 3,4-dehydroproline using initial oxidation with isatin or H2O2 and subsequent reaction with p-dimethylamino benzaldehyde (Ehrlich reagent). The method is sensitive enough to detect as low as 0.6 micrograms of 3,4-dehydroproline/sq. cm on paper chromatogram. 3,4-dehydroproline could also be estimated by the classical H2O2-oxidation procedure employed for hydroxyproline apparently yielding the same chromophore on a molar basis. However, when estimated by the chloramine-T oxidation method, its sensitivity was only 1/100th of that of 4-hydroxyproline. The usefulness of these procedures in the detection and estimation of 3,4-dehydroproline has been described.
已描述了一种检测和估算3,4-脱氢脯氨酸的方法，该方法先用异吲哚酮或过氧化氢进行初始氧化，随后与对二甲基氨基苯甲醛（埃利希试剂）反应。该方法灵敏度足够高，能够在纸色谱上检测低至0.6微克/平方厘米的3,4-脱氢脯氨酸。3,4-脱氢脯氨酸也可以通过用于羟脯氨酸的经典过氧化氢氧化程序进行估算，显然在摩尔基础上产生相同的发色团。然而，当通过氯胺-T氧化法进行估算时，其灵敏度仅为4-羟脯氨酸的1/100。已描述了这些程序在检测和估算3,4-脱氢脯氨酸方面的实用性。
