Li Yong, Chen Youliang, Li Hua
College of Animal Sciences, Zhejiang University, Hangzhou 310058, PR China.
College of Animal Sciences, Zhejiang University, Hangzhou 310058, PR China.
Ultrason Sonochem. 2017 Jan;34:281-288. doi: 10.1016/j.ultsonch.2016.05.032. Epub 2016 May 20.
Response surface methodology was used to optimize ultrasound-assisted ethanol extraction (UAE) of cholesterol from cholesterol-β-cyclodextrin (C-β-CD) inclusion complex prepared from duck yolk oil. The best extraction conditions were solvent-solid ratio 10mL/g, ultrasonic power 251W, extraction temperature 56°C and sonication time 36min. Under these conditions, the highest cholesterol extraction yield and cholesterol content obtained 98.12±0.25% and 43.38±0.61mg/g inclusion complex, respectively. As compared with Reflux extraction and Soxhlet extraction, the UAE was more efficient and economical. To increase the purity of crude cholesterol extraction, silica gel column chromatography and crystallization were carried out. Finally, cholesterol was obtained at 95.1% purity, 71.7% recovery and 22.0% yield.
采用响应面法对从鸭蛋黄油制备的胆固醇-β-环糊精（C-β-CD）包合物中超声辅助乙醇提取（UAE）胆固醇的工艺进行优化。最佳提取条件为料液比10mL/g、超声功率251W、提取温度56℃、超声时间36min。在此条件下，胆固醇的最高提取率和包合物中胆固醇含量分别为98.12±0.25%和43.38±0.61mg/g。与回流提取和索氏提取相比，超声辅助提取更高效、经济。为提高粗胆固醇提取物的纯度，进行了硅胶柱色谱和结晶操作。最终得到的胆固醇纯度为95.1%，回收率为71.7%，产率为22.0%。
